Degradation and osteogenic induction of a SrHPO4-coated Mg-Nd-Zn-Zr alloy intramedullary nail in a rat femoral shaft fracture model

Zhe Wang; Xinyuan Wang; Jia Pei; Yuan Tian; Jian Zhang; Chang Jiang; Junming Huang; Zhiying Pang; Yuanwu Cao; Xiuhui Wang; Senbo An; Xiao Wang; Hua Huang; Guangyin Yuan; Zuoqin Yan

doi:10.1016/j.biomaterials.2020.119962

Degradation and osteogenic induction of a SrHPO₄-coated Mg-Nd-Zn-Zr alloy intramedullary nail in a rat femoral shaft fracture model

Biomaterials. 2020 Jul:247:119962. doi: 10.1016/j.biomaterials.2020.119962. Epub 2020 Mar 31.

Authors

Zhe Wang¹, Xinyuan Wang², Jia Pei³, Yuan Tian⁴, Jian Zhang⁵, Chang Jiang², Junming Huang², Zhiying Pang², Yuanwu Cao², Xiuhui Wang⁶, Senbo An⁷, Xiao Wang⁸, Hua Huang⁹, Guangyin Yuan¹⁰, Zuoqin Yan¹¹

Affiliations

¹ Department of Orthopedics, Zhongshan Hospital, Fudan University, Shanghai, 200032, China; Department of Orthopedic Surgery, School of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA.
² Department of Orthopedics, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
³ National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, Shanghai Jiao Tong University, Shanghai, 200240, China; Shanghai Innovation Institute for Materials, Shanghai, 200444, China.
⁴ National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, Shanghai Jiao Tong University, Shanghai, 200240, China.
⁵ Shanghai Innovation Medical Technology Co., Ltd, Shanghai, 201306, China.
⁶ Department of Orthopedics Zhoupu Hospital Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai, 201318, China.
⁷ Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, 410008, China.
⁸ Department of Orthopedic Surgery, School of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA.
⁹ National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, Shanghai Jiao Tong University, Shanghai, 200240, China; Shanghai Innovation Institute for Materials, Shanghai, 200444, China. Electronic address: huangh@sjtu.edu.cn.
¹⁰ National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, Shanghai Jiao Tong University, Shanghai, 200240, China; Shanghai Innovation Institute for Materials, Shanghai, 200444, China. Electronic address: gyyuan@sjtu.edu.cn.
¹¹ Department of Orthopedics, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. Electronic address: Yan.zuoqin@zs-hospital.sh.cn.

PMID: 32251929
DOI: 10.1016/j.biomaterials.2020.119962

Abstract

Magnesium and Mg-based alloys are promising biomaterials for orthopedic implants because of their degradability, osteogenic effects, and biocompatibility. However, the drawbacks of these materials include high hydrogen gas production, unexpected corrosion resistance, and insufficient mechanical strength duration. Surface modification can protect these biomaterials and induce osteogenesis. In this work, a SrHPO₄ coating was developed for our patented biodegradable Mg-Nd-Zn-Zr alloy (abbr. JDBM) through a chemical deposition method. The coating was characterized by in vitro immersion, ion release, and cytotoxicity tests, which showed a slower corrosion behavior and excellent cell viability. RNA sequencing of MC3T3E1 cells treated with SrHPO₄-coated JDBM ion release test extract showed increased Tlr4, followed by the activation of the downstream PI3K/Akt signaling pathway, causing proliferation and growth of pre-osteoblasts. An intramedullary nail (IMN) was implanted in a femoral fracture rat model. Mechanical test, radiological and histological analysis suggested that SrHPO₄-coated JDBM has superior mechanical properties, induces more bone formation, and decreases the degradation rate compared with uncoated JDBM and the administration of TLR4 inhibitor attenuated the new bone formation for fracture healing. SrHPO₄ is a promising coating for JDBM implants, particularly for long-bone fractures.

Keywords: Coating; Intramedullary nail; JDBM; MC3T3E1; Strontium hydrogen phosphate; TLR4.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alloys
Animals
Coated Materials, Biocompatible
Corrosion
Femoral Fractures*
Materials Testing
Osteogenesis*
Phosphatidylinositol 3-Kinases
Rats

Substances

Alloys
Coated Materials, Biocompatible
Mg-Nd-Zn-Zr alloy