Analysis of bisphosphonate using ultraviolet-visible spectroscopy based on modified phosphorus determination reagent

Jinyu Wang; Yaping Wu; Jiawei Jiang; Guanxiong Liu; Peizhi Zhu

doi:10.1016/j.saa.2020.118308

Analysis of bisphosphonate using ultraviolet-visible spectroscopy based on modified phosphorus determination reagent

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Jul 5:235:118308. doi: 10.1016/j.saa.2020.118308. Epub 2020 Mar 26.

Authors

Jinyu Wang¹, Yaping Wu¹, Jiawei Jiang¹, Guanxiong Liu¹, Peizhi Zhu²

Affiliations

¹ Institution of Biomedicine and Tissue Engineering, School of Chemistry and Chemical Engineering, Yangzhou University, Jiangsu 225002, PR China.
² Institution of Biomedicine and Tissue Engineering, School of Chemistry and Chemical Engineering, Yangzhou University, Jiangsu 225002, PR China. Electronic address: pzua@163.com.

PMID: 32251892
DOI: 10.1016/j.saa.2020.118308

Abstract

An improved phosphorous determination was developed using ethanol, phosphorus determination reagent (PDR) and Ultraviolet-visible spectroscopy (UV-Vis) for analyzing the bisphosphonates (BPs). The method was carried out under mild conditions without digestion, high temperature, high pressure, and other extreme conditions. Alcohols played an important role in this method. Without alcohol, this reaction system did not have a color reaction. Alendronate (ALN) and risedronate (RIS) were used to demonstrate the reliability of the improved phosphorous determination under different reaction conditions. The absorbance had an equal ratio of increase as well as a good trend line when the content of BPs increased. The improved phosphorous determination could be a new method to measure the drug content of BPs.

Keywords: Alendronate; Bisphosphonate; Phosphorous determination; Risedronate; UV–Vis.