A TaqMan probe based real-time PCR for the detection of Decapod iridescent virus 1

Liang Qiu; Xing Chen; Xiao-Meng Guo; Wen Gao; Ruo-Heng Zhao; Qing-Li Zhang; Bing Yang; Jie Huang

doi:10.1016/j.jip.2020.107367

A TaqMan probe based real-time PCR for the detection of Decapod iridescent virus 1

J Invertebr Pathol. 2020 Jun:173:107367. doi: 10.1016/j.jip.2020.107367. Epub 2020 Apr 3.

Authors

Liang Qiu¹, Xing Chen¹, Xiao-Meng Guo², Wen Gao³, Ruo-Heng Zhao¹, Qing-Li Zhang¹, Bing Yang¹, Jie Huang⁴

Affiliations

¹ Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
² Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
³ Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Shanghai Ocean University, Shanghai 201306, China.
⁴ Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China; Shanghai Ocean University, Shanghai 201306, China. Electronic address: huangjie@ysfri.ac.cn.

PMID: 32251642
DOI: 10.1016/j.jip.2020.107367

Abstract

Decapod iridescent virus 1 (DIV1) was proven to be the aetiological agent of a disease causing mass die-offs of shrimp, prawn and crayfish. The specific purpose of this study was to develop a new sensitive real-time PCR method for the specific detection of DIV1. A pair of primers that amplify a 142 bp fragment and a TaqMan probe were selected for the major capsid protein gene of DIV1. They were shown to be specific for DIV1 and did not react with other common shrimp pathogens or healthy shrimp DNA. The method could detect as virus levels as low as 1.2 copies of DIV1 plasmid DNA.

Keywords: Cherax quadricarinatus iridovirus; Decapod iridescent virus 1; Real-time PCR; Shrimp; Shrimp hemocyte iridescent virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Iridoviridae / isolation & purification*
Penaeidae / virology*
Real-Time Polymerase Chain Reaction / instrumentation
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Viral Load