Peroxidase as a simultaneous degradation agent of ochratoxin A and zearalenone applied to model solution and beer

Abstract

The aim of this study was to evaluate the action of the commercial peroxidase (POD) enzyme (Armoracia rusticana) on the simultaneous degradation of ochratoxin A (OTA) and zearalenone (ZEA) in model solution and beer. For this purpose, the reaction parameters for POD action were optimized, POD application in the degradation of mycotoxins in model solution and beer was evaluated and the kinetic parameters of POD were defined (Michaelis-Menten constant - K_M and maximal velocity - V_max). In the reaction conditions (pH 7, ionic strength of 25 mM, incubation at 30 °C, addition of 26 mM H₂O₂ and 1 mM potassium ion), POD (0.6 U mL^-1) presented the maximum activity for simultaneous degradation of OTA and ZEA of 27.0 and 64.9%, respectively, in model solution after 360 min. The application of POD in beer resulted in the simultaneous degradation of OTA and ZEA of 4.8 and 10.9%, respectively. The kinetic parameters K_M and V_max for degradation of OTA and ZEA were 50 and 10,710 nM and 0.168 and 72 nM min^-1, respectively. Therefore, POD can be a promising alternative to mitigate the contamination of OTA and ZEA in model solution and beer, minimizing their effects in humans.

Keywords: Armoracia rusticana; Biological degradation; Enzymatic action; Enzymatic kinetics; Enzymatic optimization; Mycotoxin; Ochratoxin A; Zearalenone.