Desalted duck egg white peptides-chitosan oligosaccharide copolymers as calcium delivery systems: Preparation, characterization and calcium release evaluation in vitro and vivo

Food Res Int. 2020 May:131:108974. doi: 10.1016/j.foodres.2019.108974. Epub 2020 Jan 21.

Abstract

Two novel calcium delivery systems with high calcium-binding ability were prepared with desalted duck egg white peptides (DPs) and chitosan oligosaccharide (COS) by Amadori-type linkage and transglutaminase (TGase) induced reaction. Fourier transform infrared spectroscopy (FTIR), Zeta potential and particle size analyses were used to characterize the copolymers. These two copolymers reversed the inhibition of phytic acid (PA) in calcium transport in Caco-2 cell monolayers. PA-induced calcium deficiency mice model indicated that copolymers could reverse the side effect of PA, promote calcium bioavailability, and improve gut health. Overall, these results confirmed the potentialities of DPs-COS copolymers as nutraceuticals/functional food for oral calcium delivery to promote calcium absorption and improve gut health.

Keywords: Calcium delivery system; Chitosan oligosaccharide (COS); Desalted duck egg white peptides (DPs); Phytic acid-induced calcium deficiency; Structural characterization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport
  • Caco-2 Cells
  • Calcium / administration & dosage*
  • Chitosan / chemistry*
  • Chitosan / metabolism*
  • Drug Liberation
  • Ducks
  • Egg Proteins / chemistry*
  • Humans
  • Male
  • Mice
  • Nanostructures
  • Oligosaccharides / chemistry*
  • Polymers / chemistry

Substances

  • Egg Proteins
  • Oligosaccharides
  • Polymers
  • Chitosan
  • Calcium