Effect of scopoletin on phagocytic activity of U937-derived human macrophages: Insights from transcriptomic analysis

Amgad I Alkorashy; Ahmed S Doghish; Ahmed I Abulsoud; Mohamed G Ewees; Tamer M Abdelghany; Mostafa M Elshafey; Walid F Elkhatib

doi:10.1016/j.ygeno.2020.03.022

Effect of scopoletin on phagocytic activity of U937-derived human macrophages: Insights from transcriptomic analysis

Genomics. 2020 Sep;112(5):3518-3524. doi: 10.1016/j.ygeno.2020.03.022. Epub 2020 Mar 31.

Authors

Amgad I Alkorashy¹, Ahmed S Doghish², Ahmed I Abulsoud³, Mohamed G Ewees⁴, Tamer M Abdelghany⁴, Mostafa M Elshafey¹, Walid F Elkhatib⁵

Affiliations

¹ Department of Biochemistry, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo 11651, Egypt.
² Department of Biochemistry, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo 11651, Egypt; Department of Biochemistry, Faculty of Pharmacy, Badr University in Cairo (BUC), Badr City, Cairo 11829, Egypt. Electronic address: ahmed_doghish@azhar.edu.eg.
³ Department of Biochemistry, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo 11651, Egypt; Department of Biochemistry and Biotechnology, Faculty of Pharmacy, Heliopolis University, Cairo 11785, Egypt.
⁴ Department of Pharmacology and Toxicology, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo 11651, Egypt; Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
⁵ Department of Microbiology and Immunology, Faculty of Pharmacy, Badr University in Cairo (BUC), Badr City, Cairo 11829, Egypt; Microbiology and Immunology Department, Faculty of Pharmacy, Ain Shams University, African Union Organization St., Abbassia, Cairo 11566, Egypt. Electronic address: walid-elkhatib@pharma.asu.edu.eg.

PMID: 32243896
DOI: 10.1016/j.ygeno.2020.03.022

Abstract

Scopoletin is a botanical coumarin. Notably, scopoletin effect on phagocytic activity has not been addressed on transcriptomic level. Accordingly, this study investigated the effect of scopoletin on phagocytosis-linked gene transcription. Whole phagocytosis transcriptional profiling of stimulated U937-derived macrophages (SUDMs) in response to scopoletin as compared to non-treated SUDMs was studied. Regarding scopoletin effect on 92 phagocytosis-linked genes, 12 of them were significantly affected (p-value < .05). Seven genes were downregulated (CDC42, FCGR1A/FCGR1C, ITGA9, ITGB3, PLCE1, RHOD & RND3) and five were upregulated (DIRAS3, ITGA1, PIK3CA, PIK3R3 & PLCD1). Moreover, scopoletin enhanced phagocytic activity of SUDMs. The current results highlighted the potential use of scopoletin as immunity booster and as an adjuvant remedy in management of some autoimmune reactions. To the best of our knowledge, this is the first report that unravels the effect of scopoletin on phagocytosis via transcriptomic analysis.

Keywords: Immunity booster; Macrophages; Phagocytosis; Scopoletin; Transcriptomic; U937-derived macrophages.

MeSH terms

Gene Expression Profiling
Humans
Macrophage Activation / drug effects
Macrophages / drug effects*
Macrophages / immunology
Macrophages / metabolism
Phagocytosis / drug effects*
Scopoletin / pharmacology*
Transcriptome / drug effects*
U937 Cells

Substances

Scopoletin