Bradford Assay for Determining Protein Concentration

Cold Spring Harb Protoc. 2020 Apr 1;2020(4):102269. doi: 10.1101/pdb.prot102269.

Abstract

The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.

MeSH terms

  • Animals
  • Cattle
  • Colorimetry / methods*
  • Immunoglobulin G / analysis*
  • Immunoglobulin G / chemistry
  • Proteins / analysis*
  • Proteins / chemistry
  • Proteins / standards
  • Reference Standards
  • Reproducibility of Results
  • Rosaniline Dyes / chemistry*
  • Serum Albumin, Bovine / analysis*
  • Serum Albumin, Bovine / chemistry
  • Spectrophotometry / methods

Substances

  • Immunoglobulin G
  • Proteins
  • Rosaniline Dyes
  • Serum Albumin, Bovine
  • coomassie Brilliant Blue