The main objective of the present paper is the green synthesis of colloidal silver by ultrasonication starting from silver nitrate and using soluble starch as the reducing agent. Soluble starch has been used during synthesis because it is a cheap and environmentally friendly reactive. Silver colloid has been characterized by physicochemical methods: UV-VIS spectroscopy, Scanning Electron Microscopy and Energy Dispersive X-Ray spectroscopy. This colloidal material was prepared in order to prove and establish its toxicity on heterotrophic bacteria. Toxicity tests were carried out using test cultures with and without silver colloid with different concentrations. This way was possible to establish the minimum silver concentration that presents a toxic effect against used bacteria. Quantitative evaluation of bacterial growth was performed by using the Most Probable Number method. By counting the bacterial colony number, the antibacterial effect was determined for colloidal silver deposited onto the cotton gauze by adsorption. During the present study, we optimized the adsorption specific parameters: solid:liquid ratio, temperature, contact time, colloidal silver concentration. By thermodynamic, equilibrium and kinetic studies, the adsorptive process mechanism was established.
Keywords: adsorption; antibacterial effect; band aid; colloidal silver; soluble starch; ultrasonic.