Increased autophagic activity in roots caused by overexpression of the autophagy-related gene MdATG10 in apple enhances salt tolerance

Abstract

Autophagy is a conserved pathway to degrade and recycle damaged proteins and organelles, which has generally been reported to play an important role in plant adaption to various abiotic stressors. Here, we isolated a new apple autophagy-related gene, MdATG10, from Malus domestica. Expression of MdATG10 was induced by salt stress, particularly in roots. To investigate the effects of increased autophagic activity on salt tolerance of apple, we generated three MdATG10-overexpressing apple lines and exposed them to salt stress. The transgenic apple plants exhibited enhanced salt tolerance, accompanied by slightly damaged photosynthetic ability and a milder growth limitation under the salt treatment. In addition, damage to growth and vitality of the root system caused by the salt treatment was alleviated by overexpressing MdATG10. Furthermore, reduced accumulation of Na⁺ and a lower Na⁺: K⁺ ratio was detected in the MdATG10-overexpressing apple lines under salt stress. The salt treatment induced expression of genes involved in ion homeostasis in transgenic apple roots. These results demonstrate a promoting role of autophagy in ion transport when plants encounter salty conditions.

Keywords: Apple; Autophagy; Ion homeostasis; MdATG10; Root; Salt stress.