Fullerol C60(OH)24 Nanoparticles Affect Secondary Metabolite Profile of Important Foodborne Mycotoxigenic Fungi In Vitro

Tihomir Kovač; Bojan Šarkanj; Ivana Borišev; Aleksandar Djordjevic; Danica Jović; Ante Lončarić; Jurislav Babić; Antun Jozinović; Tamara Krska; Johann Gangl; Chibundu N Ezekiel; Michael Sulyok; Rudolf Krska

doi:10.3390/toxins12040213

Fullerol C₆₀(OH)₂₄ Nanoparticles Affect Secondary Metabolite Profile of Important Foodborne Mycotoxigenic Fungi In Vitro

Toxins (Basel). 2020 Mar 27;12(4):213. doi: 10.3390/toxins12040213.

Authors

Tihomir Kovač^{1

2}, Bojan Šarkanj^{1

2

3}, Ivana Borišev⁴, Aleksandar Djordjevic⁴, Danica Jović⁴, Ante Lončarić¹, Jurislav Babić¹, Antun Jozinović¹, Tamara Krska², Johann Gangl⁵, Chibundu N Ezekiel^{2

6}, Michael Sulyok², Rudolf Krska^{2

7}

Affiliations

¹ Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, 31000 Osijek, Croatia.
² Institute of Bioanalytics and Agro-Metabolomics, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenzstr. 20, 3430 Tulln, Austria.
³ Department of Food Technology, University North, Trg dr. Žarka Dolinara 1, 48000 Koprivnica, Croatia.
⁴ Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia.
⁵ Institute of Biotechnology in Plant Production, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenzstr. 20, 3430 Tulln, Austria.
⁶ Department of Microbiology, Babcock University, Ilishan Remo 121103, Ogun State, Nigeria.
⁷ Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, University Road, Belfast BT7 1NN, Northern Ireland, UK.

Abstract

Despite the efforts to control mycotoxin contamination worldwide, extensive contamination has been reported to occur in food and feed. The contamination is even more intense due to climate changes and different stressors. This study examined the impact of fullerol C₆₀(OH)₂₄ nanoparticles (FNP) (at 0, 1, 10, 100, and 1000 ng mL^-1) on the secondary metabolite profile of the most relevant foodborne mycotoxigenic fungi from genera Aspergillus, Fusarium, Alternaria and Penicillium, during growth in vitro. Fungi were grown in liquid RPMI 1640 media for 72 h at 29 °C, and metabolites were investigated by the LC-MS/MS dilute and shoot multimycotoxin method. Exposure to FNP showed great potential in decreasing the concentrations of 35 secondary metabolites; the decreases were dependent on FNP concentration and fungal genus. These results are a relevant guide for future examination of fungi-FNP interactions in environmental conditions. The aim is to establish the exact mechanism of FNP action and determine the impact such interactions have on food and feed safety.

Keywords: Alternaria spp.; Aspergillus spp.; Fusarium spp.; Penicillium spp.; foodborne mycotoxigenic fungi; fullerol C60(OH)24; mycotoxins; nanoparticles; secondary metabolism.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Alternaria / drug effects
Alternaria / growth & development
Alternaria / metabolism
Aspergillus / drug effects
Aspergillus / growth & development
Aspergillus / metabolism
Food Microbiology*
Foodborne Diseases / microbiology
Foodborne Diseases / prevention & control*
Fullerenes / pharmacology*
Fungicides, Industrial / pharmacology*
Fusarium / drug effects
Fusarium / growth & development
Fusarium / metabolism
Mitosporic Fungi / drug effects*
Mitosporic Fungi / growth & development
Mitosporic Fungi / metabolism
Nanoparticles*
Penicillium / drug effects
Penicillium / growth & development
Penicillium / metabolism
Secondary Metabolism / drug effects*
Species Specificity

Substances

Fullerenes
Fungicides, Industrial
fullerol C60(OH)24