Surface LSP-1 Is a Phenotypic Marker Distinguishing Human Classical versus Monocyte-Derived Dendritic Cells

Sandrine Moutel; Anne Beugnet; Aurélie Schneider; Bérangère Lombard; Damarys Loew; Sebastian Amigorena; Franck Perez; Elodie Segura

doi:10.1016/j.isci.2020.100987

Surface LSP-1 Is a Phenotypic Marker Distinguishing Human Classical versus Monocyte-Derived Dendritic Cells

iScience. 2020 Apr 24;23(4):100987. doi: 10.1016/j.isci.2020.100987. Epub 2020 Mar 16.

Authors

Sandrine Moutel¹, Anne Beugnet¹, Aurélie Schneider¹, Bérangère Lombard², Damarys Loew², Sebastian Amigorena³, Franck Perez¹, Elodie Segura⁴

Affiliations

¹ Institut Curie, PSL Research University, CNRS, UMR144, 26 rue d'Ulm, 75005 Paris, France.
² Institut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, 26 rue d'Ulm, 75005 Paris, France.
³ Institut Curie, PSL Research University, INSERM, U932, 26 rue d'Ulm, 75005 Paris, France.
⁴ Institut Curie, PSL Research University, INSERM, U932, 26 rue d'Ulm, 75005 Paris, France. Electronic address: elodie.segura@curie.fr.

Abstract

Human mononuclear phagocytes comprise several subsets of dendritic cells (DCs), monocytes, and macrophages. Distinguishing one population from another is challenging, especially in inflamed tissues, owing to the promiscuous expression of phenotypic markers. Using a synthetic library of humanized llama single domain antibodies, we identified a novel surface marker for human naturally occurring monocyte-derived DCs. Our antibody targets an extra-cellular domain of LSP-1, specifically on monocyte-derived DCs, but not on other leukocytes, in particular monocytes, macrophages, classical DCs, or the recently described blood DC3 population. Our findings will pave the way for a better characterization of human mononuclear phagocytes in pathological settings.

Keywords: Molecular Biology.