EphA7 is required for otic epithelial homeostasis by modulating Claudin6 in Xenopus

Xiaolei Wang; Jian Sun; Zhaobao Wang; Chaocui Li; Bingyu Mao

doi:10.1016/j.bbrc.2020.03.099

EphA7 is required for otic epithelial homeostasis by modulating Claudin6 in Xenopus

Biochem Biophys Res Commun. 2020 May 28;526(2):375-380. doi: 10.1016/j.bbrc.2020.03.099. Epub 2020 Mar 25.

Authors

Xiaolei Wang¹, Jian Sun², Zhaobao Wang³, Chaocui Li⁴, Bingyu Mao⁵

Affiliations

¹ Medical College, Qingdao University, Qingdao, 266071, China; State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China.
² State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China; Cancer and Developmental Biology Laboratory, National Cancer Institute, National Institute of Health, Frederick, MD, 21702, USA.
³ School of Control Science and Engineering, Shandong University, Jinan, 250061, China; Energy-rich Compounds Production by Photosynthetic Carbon Fixation Research Center, College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China.
⁴ State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China.
⁵ State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China. Electronic address: mao@mail.kiz.ac.cn.

PMID: 32222280
DOI: 10.1016/j.bbrc.2020.03.099

Abstract

Receptor tyrosine kinase EphA7 is specifically expressed in otic region in Xenopus early development. However, its role in otocyst development remains unknown. Knockdown of EphA7 by a specific morpholino oligonucleotide (MO) reduced the size of the otocyst and triggered otic epithelial cell extrusion. Interestingly, EphA7 depletion attenuated the membrane level of the tight junction protein Claudin6 (CLDN6). Utilizing the Cldn6 MO, we further confirmed that CLDN6 attenuation also led to otic epithelial cell extrusion. Our work suggested that EphA7 modulates the otic epithelial homeostasis through stabilizing the CLDN6 membrane level.

Keywords: Cell extrusion; Claudin6; EphA7; Otocyst development; Xenopus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Claudins / genetics*
Gene Expression Regulation, Developmental*
Gene Knockdown Techniques
Homeostasis
Receptor, EphA7 / genetics*
Xenopus Proteins / genetics*
Xenopus laevis / embryology*
Xenopus laevis / genetics

Substances

Claudins
Xenopus Proteins
Receptor, EphA7
claudin 6