Airyscan Superresolution Microscopy to Study Trypanosomatid Cell Biology

Jane Harmer; Asma Belbelazi; Martin Carr; Michael L Ginger

doi:10.1007/978-1-0716-0294-2_27

Airyscan Superresolution Microscopy to Study Trypanosomatid Cell Biology

Methods Mol Biol. 2020:2116:449-461. doi: 10.1007/978-1-0716-0294-2_27.

Authors

Jane Harmer¹, Asma Belbelazi², Martin Carr², Michael L Ginger³

Affiliations

¹ Department of Biological and Geographical Sciences, School of Applied Sciences, University of Huddersfield, Huddersfield, UK. J.Harmer@hud.ac.uk.
² Department of Biological and Geographical Sciences, School of Applied Sciences, University of Huddersfield, Huddersfield, UK.
³ School of Applied Sciences, University of Huddersfield, Huddersfield, UK.

PMID: 32221936
DOI: 10.1007/978-1-0716-0294-2_27

Abstract

The recent introduction by Carl Zeiss Ltd. of the Airyscan detector module for their LSM880 confocal laser-scanning microscope has enabled routine superresolution microscopy to be combined with the advantages of confocal-based fluorescence imaging. Resulting enhanced spatial resolution in X, Y, and Z provides tractable opportunity to derive new insight into protein localization(s), organelle dynamics, and thence protein function within trypanosomatids or other organisms. Here, we describe methods for preparing slides, cells, and basic microscope setup for fluorescence imaging of trypanosomatids using the LSM-880 with Airyscan platform.

Keywords: Confocal; Crithidia; Immunofluorescence; Leishmania; Live-cell imaging; Novymonas; Superresolution microscopy; Trypanosoma; Trypanosomatids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cytoskeleton
Flagella
Fluorescent Dyes / chemistry
Intravital Microscopy / instrumentation
Intravital Microscopy / methods*
Microscopy, Confocal / instrumentation
Microscopy, Confocal / methods
Staining and Labeling / methods*
Trypanosomatina / cytology*

Substances

Fluorescent Dyes