We screened raptors (n=188) and columbids (n=2) presenting to a Tampa, Florida, US rehabilitation center from June 2016 to April 2017 for Trichomonas gallinae. One juvenile Cooper's Hawk (Accipiter cooperii) was culture- and PCR-positive and sequencing identified as genotype E1. A subset of culture-negative (10%, n=19) samples were negative via PCR.