A fluorescent nanobiosensor for the facile analysis of m6A RNA demethylase activity

Chem Commun (Camb). 2020 Apr 30;56(34):4716-4719. doi: 10.1039/c9cc10054g. Epub 2020 Mar 26.

Abstract

RNA demethylase has recently been known to be associated with cancer development but its selective inhibitors as anti-cancer agents have rarely been investigated to date. Herein, we have developed a fluorescent nanobiosensor which enables efficient quantitative analysis of RNA demethylase ALKBH5 activity and shows a high potential for robust inhibitor screening.

MeSH terms

  • AlkB Homolog 5, RNA Demethylase / chemistry*
  • Biosensing Techniques*
  • DNA-Binding Proteins / chemistry*
  • Demethylation
  • Endoribonucleases / chemistry*
  • Escherichia coli Proteins / chemistry*
  • Graphite / chemistry*
  • Nanoparticles / chemistry*
  • Spectrometry, Fluorescence

Substances

  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • MazF protein, E coli
  • graphene oxide
  • Graphite
  • AlkB Homolog 5, RNA Demethylase
  • Endoribonucleases