CCAAT/Enhancer-Binding Protein β Mediates Oxygen-Induced Retinal Neovascularization via Retinal Vascular Damage and Vascular Endothelial Growth Factor

J Diabetes Res. 2020 Mar 9:2020:2789209. doi: 10.1155/2020/2789209. eCollection 2020.

Abstract

Objective: To evaluate the role of CCAAT/enhancer-binding protein β (C/EBP β (C/EBP.

Methods: Rats with OIR were exposed to alternating hypoxic and hyperopic conditions for 14 days. Then, the rats with OIR were assigned randomly to groups that received intravitreal injections of either shRNA lentiviral particles targeting C/EBP β (C/EBP β (C/EBP β (C/EBP β (C/EBP β (C/EBP β (C/EBP.

Results: In OIR rats, the expression levels of C/EBP β (C/EBP P < 0.01). The p-C/EBP β (C/EBP β (C/EBP β (C/EBP β (C/EBP β (C/EBP P < 0.01). The p-C/EBP β (C/EBP β (C/EBP β (C/EBP P < 0.01). The p-C/EBP.

Conclusions: C/EBP β shRNA inhibits RNV in OIR. A potential mechanism may be that the activity of C/EBP β increases with its overexpression, which in turn aggravates the amount of the retinal vascular damage and promotes transcription of VEGF. C/EBP β might be a new therapeutic target for preventing RNV.β (C/EBP β (C/EBP β (C/EBP.

MeSH terms

  • Animals
  • CCAAT-Enhancer-Binding Protein-beta / genetics
  • CCAAT-Enhancer-Binding Protein-beta / metabolism*
  • Disease Models, Animal
  • Gene Silencing
  • Oxygen
  • RNA, Small Interfering
  • Rats
  • Rats, Sprague-Dawley
  • Retinal Diseases / chemically induced
  • Retinal Diseases / genetics
  • Retinal Diseases / metabolism*
  • Retinal Neovascularization / genetics
  • Retinal Neovascularization / metabolism*
  • Vascular Endothelial Growth Factor A / metabolism*

Substances

  • CCAAT-Enhancer-Binding Protein-beta
  • RNA, Small Interfering
  • Vascular Endothelial Growth Factor A
  • Oxygen