Preimplantation Genetic Testing of Aneuploidy by Next Generation Sequencing: Association of Maternal Age and Chromosomal Abnormalities of Blastocyst

Tien-Truong Dang; Thi-Mui Phung; Hoang Le; Thi-Bich-Van Nguyen; Thi-Sim Nguyen; Thi-Lien-Huong Nguyen; Vu Thi Nga; Dinh-Toi Chu; Van-Luong Hoang; Duy-Bac Nguyen

doi:10.3889/oamjms.2019.875

Preimplantation Genetic Testing of Aneuploidy by Next Generation Sequencing: Association of Maternal Age and Chromosomal Abnormalities of Blastocyst

Open Access Maced J Med Sci. 2019 Dec 20;7(24):4427-4431. doi: 10.3889/oamjms.2019.875. eCollection 2019 Dec 30.

Authors

Affiliations

¹ Vietnam Military Medical University, Hanoi, Vietnam.
² Tam Anh Hospital, Hanoi, Vietnam.
³ National Hospital of Obstetrics and Gynecology, Hanoi, Vietnam.
⁴ Hanoi Obstetrics and Gynecology Hospital, Hanoi, Vietnam.
⁵ Institute for Research and Development, Duy Tan University, Danang 550000, Vietnam.
⁶ Faculty of Biology, Hanoi National University of Education, Hanoi, Vietnam.
⁷ School of Odonto Stomatology, Hanoi Medical University, Hanoi, Vietnam.

Abstract

Background: Aneuploidy is a major cause of miscarriages and implantation failure. Preimplantation genetic testing for aneuploidy (PGT-A) by Next Generation Sequencing (NGS) is able to detect of the numeral and structural chromosomal abnormalities of embryos in vitro fertilization (IVF).

Aim: This study was aimed to assess the relationship between maternal age and chromosomal abnormalities NGS technology.

Methods: A group of 603 human trophectoderm (TE) biopsied samples were tested by Veriseq kit of Illumina. The relation of marternal age and chromosomal abnormality of blastocyst embryo was evaluated.

Results: Among the 603 TE samples, 247 samples (42.73%) presented as chromosomal abnormalities. The abnormalities occurred to almost chromosomes, and the most popular aneuploidy observed is 22. Aneuploidy rate from 0.87% in chromosome 11 to 6.06% in chromosome 22. The rate of abnormal chromosome increased dramatically in group of mother's ages over 37 (54.17%) comparing to group of mother's ages less than 37 (38.05%) (p < 0.000). The Abnormal chromosome and maternal age has a positive correlation with r = 0.4783 (p<0.0001).

Conclusion: These results showed high rate abnormal chromosome and correlated with advanced maternal age of blastocyst embryos.

Keywords: Aneuploidy; Blastocyst embryos; In vitro fertilization; Next-generation sequencing; Preimplantation genetic testing aneuploidy.