Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain

Haixue Zheng; Xiangtao Liu; Youjun Shang; Jinyan Wu; Xingwen Bai; Ye Jin; Shiqi Sun; Huichen Guo; Hong Tian; Xia Feng; Shuanghui Yin; Jianhong Guo; Guozheng Cong; Zaixin Liu; Huiyun Chang; Junwu Ma; Qingge Xie

doi:10.1007/s11434-006-2095-z

Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain

Chin Sci Bull. 2006;51(17):2072-2078. doi: 10.1007/s11434-006-2095-z.

Authors

Haixue Zheng^{1

2}, Xiangtao Liu^{1

2}, Youjun Shang¹, Jinyan Wu¹, Xingwen Bai¹, Ye Jin¹, Shiqi Sun^{1

2}, Huichen Guo¹, Hong Tian¹, Xia Feng¹, Shuanghui Yin¹, Jianhong Guo^{1

2}, Guozheng Cong^{1

2}, Zaixin Liu^{1

2}, Huiyun Chang^{1

2}, Junwu Ma^{1

2}, Qingge Xie^{1

2}

Affiliations

¹ 1Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046 China.
² 2Graduate School, Chinese Academy of Sciences, Beijing, 100081 China.

Abstract

The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK₁₂ was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In vitro transcription RNA from pSVOK₁₂ transfected IBRS-2 cells and the recovered virus RNA were isolated and sequenced, then indirect hemagglutination test, indirect immunofluorescence assays, eleectron microscope test, 50% tissue culture infecting dose (TCID₅₀) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. The result showed that the infectious clones we obtained and the virus derived from pSVOK₁₂ had the same biological properties as the parental strain HK/70. The full-length infectious cDNA clone, pSVOK₁₂, will be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and replication of SVDV.

Keywords: full-length cDNA; infectious clone; pSVOK12; reverse genetics; swine vesicular disease virus.