Polydopamine-coated nucleic acid nanogel for siRNA-mediated low-temperature photothermal therapy

Fei Ding; Xihui Gao; Xiangang Huang; Huan Ge; Miao Xie; Jiwen Qian; Jie Song; Yuehua Li; Xinyuan Zhu; Chuan Zhang

doi:10.1016/j.biomaterials.2020.119976

Polydopamine-coated nucleic acid nanogel for siRNA-mediated low-temperature photothermal therapy

Biomaterials. 2020 Jul:245:119976. doi: 10.1016/j.biomaterials.2020.119976. Epub 2020 Mar 16.

Authors

Fei Ding¹, Xihui Gao², Xiangang Huang¹, Huan Ge¹, Miao Xie¹, Jiwen Qian¹, Jie Song³, Yuehua Li⁴, Xinyuan Zhu¹, Chuan Zhang⁵

Affiliations

¹ School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.
² Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Fudan University, 131 Dong An Road, Shanghai, 200032, China.
³ Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.
⁴ Department of Radiology, Shanghai Jiao Tong University Affliated Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600, Yi Shan Rd, Shanghai, 200233, China. Electronic address: liyuehua312@163.com.
⁵ School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. Electronic address: chuanzhang@sjtu.edu.cn.

PMID: 32213362
DOI: 10.1016/j.biomaterials.2020.119976

Abstract

Photothermal therapy (PTT) normally requires to maintain the temperature of tumor lesions above 50 °C, which potentially induces local inflammation and tumor metastasis. To avoid these side effects, it is vital to achieve effective antitumor efficacy at relatively low temperature (42-45 °C) during the PTT treatment. Herein, we design a polydopamine (PDA)-coated nucleic acid nanogel as a therapeutic complex for siRNA-mediated low-temperature PTT. First, siRNAs that target the heat-shock-protein 70 (Hsp70) serve as crosslinkers to guide the DNA-grafted polycaprolactone (DNA-g-PCL) assemble into nanosized hydrogel particles through nucleic acid hybridization. Thereafter, the obtained siRNA-embedded nanogels are further coated with a thin layer of polydopamine, which not only protects the nanogels against enzymatic degradation but also endows the nanogels with excellent photothermal conversion capacity under near infrared (NIR) light irradiation. After surface PEGylation, this triple shield siRNA delivery complex shows the capability of effective ablating the tumor under relatively mild condition.

Keywords: Gene silencing; Low temperature; Photothermal therapy; Polydopamine coating; RNase resistance; siRNA delivery.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Hyperthermia, Induced*
Indoles
Nanogels
Nucleic Acids*
Phototherapy
Photothermal Therapy
Polyethylene Glycols
Polyethyleneimine
Polymers
RNA, Small Interfering
Temperature

Substances

Indoles
Nanogels
Nucleic Acids
Polymers
RNA, Small Interfering
polydopamine
polyethylene glycol polyethyleneimine nanogel
Polyethylene Glycols
Polyethyleneimine