Up-Regulation of Hsa_circ_0008792 Inhibits Osteosarcoma Cell Invasion and Migration and Promotes Apoptosis by Regulating Hsa-miR-711/ZFP1

Lu Chen; Yu Shan; Hengshuo Zhang; Hongliang Wang; Yunzhen Chen

doi:10.2147/OTT.S239256

Up-Regulation of Hsa_circ_0008792 Inhibits Osteosarcoma Cell Invasion and Migration and Promotes Apoptosis by Regulating Hsa-miR-711/ZFP1

Onco Targets Ther. 2020 Mar 11:13:2173-2181. doi: 10.2147/OTT.S239256. eCollection 2020.

Authors

Lu Chen¹, Yu Shan¹, Hengshuo Zhang¹, Hongliang Wang¹, Yunzhen Chen¹

Affiliation

¹ Department of Spine Surgery, Qilu Hospital, Shandong University, Jinan, Shandong Province 250012, Republic of China.

Abstract

Objective: Circular RNA is a newly discovered non-coding RNA. It plays an important role in regulating gene expression, and may take part in tumor progression. This study aimed to investigate the functions of hsa_circ_0008792 in osteosarcoma regulation.

Methods: We identified a circular RNA, hsa_circ_0008792, by using bioinformatics to analyze the GSE96962 dataset. The capacities of migration and invasion were assessed by wound-healing assay and transwell Matrigel assay. The ratios of G0/G1, S, and G2/M phases in cell cycle and apoptosis were measured using flow cytometry.

Results: Hsa_circ_0008792 is expressed at low levels in osteosarcoma cells, and up-regulation of hsa_circ_0008792 could suppress osteosarcoma cell migration and invasion and promote apoptosis. This regulation is mediated by hsa-miR-711/ZFP1. The expression level of hsa_circ_0008792 showed no influence on cell cycle of osteosarcoma cells.

Conclusion: Osteosarcoma is suppressed by hsa_circ_0008792/hsa-miR-711/ZFP1 axis.

Keywords: ZFP1; ceRNA network; circular RNA; microRNA; osteosarcoma.