Effect of lithium carbonate on autophagy and proliferative activity of isolated hepatocytes

Raushan Dossymbekova; Nataliya Bgatova; Zina Tungushbayeva; Kamalidin Sharipov; Gulzhan Taneyeva; Asem Kydyrbaeva; Anastasiya Solovieva

doi:10.1016/j.bbrc.2020.03.057

Effect of lithium carbonate on autophagy and proliferative activity of isolated hepatocytes

Biochem Biophys Res Commun. 2020 Jul 23;528(2):343-346. doi: 10.1016/j.bbrc.2020.03.057. Epub 2020 Mar 21.

Authors

Raushan Dossymbekova¹, Nataliya Bgatova², Zina Tungushbayeva³, Kamalidin Sharipov⁴, Gulzhan Taneyeva⁵, Asem Kydyrbaeva⁵, Anastasiya Solovieva⁶

Affiliations

¹ Department of Biology, Institute of Natural Sciences and Geography, Abai Kazakh National Pedagogical University, Abai Kazakh National Pedagogical University, Kazakhstan. Electronic address: dos.raushan@mail.ru.
² Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Siberian Branch of Russian Academy of Sciences, Russia.
³ Department of Biology, Institute of Natural Sciences and Geography, Abai Kazakh National Pedagogical University, Abai Kazakh National Pedagogical University, Kazakhstan.
⁴ Department of Biological Chemistry, Kazakh National Medical University named after S.D. Asfendiyarov, S.D. Asfendiyarov Kazakh National Medical University, Kazakhstan.
⁵ Department of Molecular Biology and Medical Genetics, Kazakh National Medical University named after S.D. Asfendiyarov, S.D. Asfendiyarov Kazakh National Medical University, Kazakhstan.
⁶ Laboratory of Pharmacological Active Compounds Research Institute of Clinical and Experimental Lymphology, Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Siberian Branch of Russian Academy of Sciences, Russia.

PMID: 32209260
DOI: 10.1016/j.bbrc.2020.03.057

Abstract

The hepatocytes were cultivated in the presence of lithium carbonate (LC) for drugs testing or possible source for transplantation in the treatment of hereditary or terminal liver diseases. The LC, as an inducer of autophagy, is a promising drug for maintaining cell homeostasis and has a significant effect on the ultrastructural organization of hepatocyte cells. Within current investigation, new mechanisms of the biological effects of lithium and the ultrastructural analysis of the primary culture of hepatocytes were studied via flow cytofluorometry, light, and electron microscopy methods. Obtained results demonstrate the absence of the toxic effect of 5 mM of LC on the primary hepatocyte culture. In addition, LC does not block the cell cycle at the G₀/G₁ stage after 24 h of hepatocyte cultivation and promotes the preservation of their viability by 48 h of the experiment. Moreover, LC does not stimulate hepatocyte apoptosis, induces autophagy and the preserves the proliferative activity of hepatocytes.

Keywords: Autophagy; Cell cycle; Isolated hepatocytes; Lithium carbonate.

MeSH terms

Animals
Autophagy / drug effects*
Cell Cycle / drug effects
Cell Proliferation / drug effects
Cell Separation*
Cells, Cultured
Hepatocytes / cytology*
Hepatocytes / drug effects
Hepatocytes / ultrastructure
Lithium Carbonate / pharmacology*
Male
Rats, Wistar

Substances

Lithium Carbonate