Bioprinting Strategies for Secretory Epithelial Organoids

Methods Mol Biol. 2020:2140:243-249. doi: 10.1007/978-1-0716-0520-2_16.

Abstract

Novel three-dimensional (3D) biofabrication platforms can allow magnetic 3D bioprinting (M3DB) by using magnetic nanoparticles to tag cells and then spatially arrange them in 3D around magnet dots. Here, we report an M3DB methodology to generate salivary gland-like epithelial organoids from stem cells. These organoids possess a neuronal network that responds to saliva neurostimulants.

Keywords: Bioprinting; Magnetic nanoparticles; Organoids; Salivary gland; Xerostomia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bioprinting / methods*
  • Dental Pulp / cytology
  • Epithelial Cells* / metabolism
  • Humans
  • Magnetic Iron Oxide Nanoparticles
  • Multipotent Stem Cells / cytology
  • Organoids* / metabolism
  • Pluripotent Stem Cells / cytology
  • Printing, Three-Dimensional*
  • Salivary Glands* / cytology
  • Spheroids, Cellular* / metabolism
  • Tissue Engineering / methods*
  • alpha-Amylases / metabolism

Substances

  • alpha-Amylases