Production and validation of human induced pluripotent stem cell line from sporadic amyotrophic lateral sclerosis (SALS)

Jun Ma; Baofeng Feng; Desheng Kong; Jingjing He; Ruiyun Guo; Asiamah Ernest Amponsah; Wei Zhang; Shuhan Zhang; Fei Lv; Yongzhou Song; Aijing Liu; Huixian Cui

doi:10.1016/j.scr.2020.101760

Production and validation of human induced pluripotent stem cell line from sporadic amyotrophic lateral sclerosis (SALS)

Stem Cell Res. 2020 Apr:44:101760. doi: 10.1016/j.scr.2020.101760. Epub 2020 Mar 13.

Authors

Affiliations

¹ Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei 050017, China; Human Anatomy Department, Hebei Medical University, Hebei 050017, China.
² Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei 050017, China.
³ Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; The Second Hospital of Hebei Medical University, Hebei 050017, China.
⁴ Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei 050017, China; Human Anatomy Department, Hebei Medical University, Hebei 050017, China. Electronic address: huixiancuihmu@163.com.

PMID: 32203916
DOI: 10.1016/j.scr.2020.101760

Abstract

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease with the loss of upper motor neurons in the cortex and lower motor neurons in the brain stem and spinal cord regressively. The vast majority of ALS cases have no familial history are apparently sporadic (SALS), making the modeling of SALS essential to the development of ALS therapeutics. Therefore, human induced pluripotent stem cell (iPSC) from peripheral blood mononuclear cells of a 64-year-old SALS patient were produced using a virus-free protocol and characterized using standard validate methods. This generated iPSC line could be useful to reveal SALS mechanisms and screen drug development.

Keywords: differentiation; induced pluripotent stem cell; reprogramming; sporadic amyotrophic lateral sclerosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amyotrophic Lateral Sclerosis* / genetics
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear
Middle Aged
Motor Neurons
Neurodegenerative Diseases*