Two new 19-hydroxy bufadienolides with cytotoxic activity from the skins of Bufo melanosticus

Lingjie Meng; Sanhua Li; Qinghong Kong; Mei Wang; Xiaoke Zhang; Xinting Zhu; Wei Yu; Nian Jiang; Zeli Chun; Nian Li; Yun Liu

doi:10.1080/14786419.2020.1741582

Two new 19-hydroxy bufadienolides with cytotoxic activity from the skins of Bufo melanosticus

Nat Prod Res. 2021 Dec;35(23):4894-4900. doi: 10.1080/14786419.2020.1741582. Epub 2020 Mar 23.

Authors

Lingjie Meng^{1

2

3}, Sanhua Li^{1

2}, Qinghong Kong^{1

2}, Mei Wang^{1

2}, Xiaoke Zhang^{1

2}, Xinting Zhu^{1

3}, Wei Yu^{1

2}, Nian Jiang^{1

2}, Zeli Chun², Nian Li¹, Yun Liu^{1

2

3}

Affiliations

¹ Guizhou Provincial College-based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines, Zunyi Medical University, Zunyi, Guizhou, China.
² Research Center for Medicine & Biology, Zunyi Medical University, Zunyi, Guizhou, China.
³ Department of Biochemistry and Molecular Biology, Zunyi Medical University, Zunyi, Guizhou, China.

PMID: 32202160
DOI: 10.1080/14786419.2020.1741582

Abstract

Two new 19-hydroxy bufadienolides, named bufohydrolide A (1) and bufohydrolide B (2), and four known analogs (3-6) were isolated from the aqueous extracts of the skins of Bufo melanosticus. Their structures were established by spectral data analyses, such as UV, IR, 1 D/2D NMR and mass spectra. Compounds 1-6 were evaluated for their cytotoxic activity against SMMC-7721, HT-29 and A549 cells. Noteworthily, all six isolates exhibited various levels of anti-proliferative activities with IC₅₀ values ranging from 0.02 ± 0.0002 to 25 ± 0.5 μM.

Keywords: 19-hydroxy bufadienolides; aqueous extracts; cytotoxic effects; skins of B. melanosticus.

MeSH terms

Animals
Antineoplastic Agents* / pharmacology
Bufanolides* / pharmacology
Bufonidae
Molecular Structure
Skin

Substances

Antineoplastic Agents
Bufanolides