Red-Emission Probe for Ratiometric Fluorescent Detection of Bisulfite and Its Application in Live Animals and Food Samples

Fang Zhou; Huan Feng; Haibo Li; Yue Wang; Zhiqiang Zhang; Wenjun Kang; Hongmin Jia; Xinyi Yang; Qingtao Meng; Run Zhang

doi:10.1021/acsomega.0c00063

Red-Emission Probe for Ratiometric Fluorescent Detection of Bisulfite and Its Application in Live Animals and Food Samples

ACS Omega. 2020 Mar 3;5(10):5452-5459. doi: 10.1021/acsomega.0c00063. eCollection 2020 Mar 17.

Authors

Fang Zhou¹, Huan Feng¹, Haibo Li², Yue Wang¹, Zhiqiang Zhang¹, Wenjun Kang², Hongmin Jia¹, Xinyi Yang¹, Qingtao Meng¹, Run Zhang^{1

3}

Affiliations

¹ School of Chemical Engineering, University of Science and Technology Liaoning, Anshan, Liaoning 114051, China.
² Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, Department of Chemistry, Liaocheng University, Liaocheng 252059, China.
³ Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane 4072, Australia.

Abstract

Key roles of bisulfite (HSO₃ ^-) in food quality assurance and human health necessitate a reliable analytical method for rapid, sensitive, and selective detection of HSO₃ ^-. Herein, a new red-emitting ratiometric fluorescence probe, BIQ, is reported for sensitive and selective detection of HSO₃ ^- in food samples and live animals. Probe BIQ recognizes HSO₃ ^- via a 1,4-nucleophilic addition reaction. As a result of this specific reaction, emission intensities at 625 and 475 nm are dramatically changed, allowing the detection of HSO₃ ^- in a ratiometric fluorescence model in an aqueous solution. The obvious changes of solution color from pink to transparent and fluorescence color from rose-red to cyan allow the detection of HSO₃ ^- by naked eyes. Furthermore, probe BIQ has fast response in color and fluorescence (<2 min), excellent selectivity, and a low detection limit (0.29 μM), which enables its application in HSO₃ ^- detection in food samples and live organisms. The practical applications of probe BIQ are then demonstrated by the visualization of HSO₃ ^- in live animals (zebrafish and nude mouse) as well as the determination of HSO₃ ^- in white wine and sugar.