Very long-term stability of lipid biomarkers in human serum

Vladimira Muzakova; Piet K Beekhof; Eugène H J M Jansen

doi:10.1016/j.ab.2020.113695

Very long-term stability of lipid biomarkers in human serum

Anal Biochem. 2020 May 15:597:113695. doi: 10.1016/j.ab.2020.113695. Epub 2020 Mar 19.

Authors

Vladimira Muzakova¹, Piet K Beekhof², Eugène H J M Jansen³

Affiliations

¹ Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic. Electronic address: vladimira.muzakova@upce.cz.
² Centre for Health Protection, National Institute of Public Health and the Environment, PO Box 1, 3720 BA, Bilthoven, Netherlands. Electronic address: piet.beekhof@rivm.nl.
³ Centre for Health Protection, National Institute of Public Health and the Environment, PO Box 1, 3720 BA, Bilthoven, Netherlands. Electronic address: eugenejansen99@gmail.com.

PMID: 32201135
DOI: 10.1016/j.ab.2020.113695

Abstract

The assessment of the stability of biomarkers is very important for epidemiological studies. In this study, the stability of five lipid parameters (total cholesterol, triglycerides, HDL- and LDL-cholesterol and free fatty acids) has been tested in 16 human serum samples after storage at -80 °C up at time points 0, 1, 8 and 13 y. The majority of the lipid biomarkers were stable during storage conditions, except for cholesterol. The correlations between the samples were very good at time points. Therefore, long-term storage of human serum samples allows lipid biomarker determination, provided that the samples are stored at -80 °C.

Keywords: Human serum; Lipid biomarkers; Storage stability.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Biomarkers / blood
Cholesterol / blood*
Cholesterol, HDL / blood*
Cholesterol, LDL / blood*
Female
Healthy Volunteers
Humans
Male
Middle Aged
Triglycerides / blood*
Young Adult

Substances

Biomarkers
Cholesterol, HDL
Cholesterol, LDL
Triglycerides
Cholesterol