A novel fluorescence probe of Plasmodium vivax lactate dehydrogenase based on adenosine monophosphate protected bimetallic nanoclusters

Chun-Xia Zhang; Julian A Tanner; Hong-Wei Li; Yuqing Wu

doi:10.1016/j.talanta.2020.120850

A novel fluorescence probe of Plasmodium vivax lactate dehydrogenase based on adenosine monophosphate protected bimetallic nanoclusters

Talanta. 2020 Jun 1:213:120850. doi: 10.1016/j.talanta.2020.120850. Epub 2020 Feb 14.

Authors

Chun-Xia Zhang¹, Julian A Tanner², Hong-Wei Li³, Yuqing Wu¹

Affiliations

¹ State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, China.
² School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
³ State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, China. Electronic address: lihongwei@jlu.edu.cn.

PMID: 32200917
DOI: 10.1016/j.talanta.2020.120850

Abstract

Specific detection of Plasmodium vivax lactate dehydrogenase (PvLDH), an important biomarker of malaria, remains a significant challenge. Herein, adenosine monophosphate protected gold-silver bimetallic nanoclusters, Au-AgNCs@AMP were used as a specific and sensitive fluorescence probe to detect PvLDH. After optimizing, a linear response was shown over a wide concentration range (10-100 nM) and an extremely low limit of detection (LOD) at 0.10 nM (3.7 ng mL^-1) was achieved finally. Albeit the method was able to detect PvLDH sensitively, it could not discriminate different types of LDHs. Consequently, Al³⁺ was employed as an "assistant agent", which induced an assay capacity to discriminate PvLDH from other LDHs. The bimetallic nanoclusters inhibited the activity of PvLDH, suggesting it bound near the active site of PvLDH with high affinity. Zeta potential and UV-vis absorption experiments showed that electrostatic interaction was the main driving force for the interaction between the nanoclusters and PvLDH. Through chemical modification it indicated free thiol groups in PvLDH played an implant role in the interaction. Overall, the fluorescence enhancement and blue-shift were attributed to assembly-induced emission enhancement (AIEE) and hydrophobic transfer. The present study provides a simple, robust, and easy-to-perform approach to detect PvLDH with high sensitivity and selectivity, with significant potential for malaria diagnosis in the developing world.

Keywords: Assembly-induced emission enhancement; Bimetallic nanoclusters; Fluorescence enhancement; Inhibition; Plasmodium vivax lactate dehydrogenase.

MeSH terms

Adenosine Monophosphate / chemistry*
Enzyme Assays / methods*
Fluorescent Dyes / chemistry*
Gold / chemistry
Humans
L-Lactate Dehydrogenase / analysis*
Limit of Detection
Malaria, Vivax / parasitology
Metal Nanoparticles / chemistry*
Plasmodium vivax / enzymology*
Silver / chemistry

Substances

Fluorescent Dyes
Silver
Adenosine Monophosphate
Gold
L-Lactate Dehydrogenase