Transcriptome study reveals apoptosis of porcine kidney cells induced by fumonisin B1 via TNF signalling pathway

Jia Chen; Shuhua Yang; Sheng Huang; Rong Yan; Mingyang Wang; Si Chen; Jing Cai; Miao Long; Peng Li

doi:10.1016/j.fct.2020.111274

Transcriptome study reveals apoptosis of porcine kidney cells induced by fumonisin B1 via TNF signalling pathway

Food Chem Toxicol. 2020 May:139:111274. doi: 10.1016/j.fct.2020.111274. Epub 2020 Mar 18.

Authors

Jia Chen¹, Shuhua Yang², Sheng Huang³, Rong Yan⁴, Mingyang Wang⁵, Si Chen⁶, Jing Cai⁷, Miao Long⁸, Peng Li⁹

Affiliations

¹ Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2017220532@stu.syau.edu.cn.
² Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: yangshuhua0001@syau.edu.cn.
³ Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2017220530@stu.syau.edu.cn.
⁴ Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2018220520@stu.syau.edu.cn.
⁵ Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: m13940546231@163.com.
⁶ Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2017220528@stu.syau.edu.cn.
⁷ Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2018200143@stu.syau.edu.cn.
⁸ Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: longmiao@syau.edu.cn.
⁹ Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: lipeng2018@syau.edu.cn.

PMID: 32198028
DOI: 10.1016/j.fct.2020.111274

Abstract

Fumonisin B₁ (FB₁) is a mycotoxin that contaminates cash crops and has toxic effects on humans and livestock. However, the toxic effect of FB₁ is not fully understood. In this study, the apoptosis mechanism of FB₁ on porcine kidney cells (PK-15) was elucidated by transcriptome analysis. The results showed that FB₁ observably changed the expression of mRNA in PK-15 and induced the cells of apoptosis after being exposed to 106 μM FB₁ in vitro. Gene Ontology and KEGG enrichment analyses showed that FB₁ exposure increased the expressions of related mRNA in TNF signalling pathway in PK-15. To verify our bioinformatics analysis, these changes were verified by qRT-PCR and Western blot assay. Furthermore, the mRNA expressions of NF-κB and its downstream genes or proteins decreased significantly (p < 0.01) after the addition of BAY11-7082, the inhibitor of NF-κB. Therefore, for the first time, we demonstrate that FB₁ can induce apoptosis of PK-15 cells through TNF signalling pathway, and NF-κB gene is a target of FB₁ acting on the TNF signalling pathway.

Keywords: Fumonisin B(1); PK-15 cell; TNF signalling Pathway; Transcriptome.

MeSH terms

Animals
Apoptosis / drug effects*
Epithelial Cells / drug effects*
Fumonisins / toxicity*
Gene Expression Profiling
Kidney / metabolism*
Mycotoxins / toxicity
NF-kappa B / genetics
NF-kappa B / metabolism
RNA, Messenger / metabolism
Signal Transduction / drug effects*
Swine
Transcriptome*

Substances

Fumonisins
Mycotoxins
NF-kappa B
RNA, Messenger
fumonisin B1