Enhanced lipid extraction from the biodiesel-producing microalga Chlorella pyrenoidosa cultivated in municipal wastewater via Daphnia ingestion and digestion

Wanqing Ding; Wenbiao Jin; Xu Zhou; Shao-Feng Li; Renjie Tu; Song-Fang Han; Chuan Chen; Xiaochi Feng; Yan Huang

doi:10.1016/j.biortech.2020.123162

Enhanced lipid extraction from the biodiesel-producing microalga Chlorella pyrenoidosa cultivated in municipal wastewater via Daphnia ingestion and digestion

Bioresour Technol. 2020 Jun:306:123162. doi: 10.1016/j.biortech.2020.123162. Epub 2020 Mar 10.

Authors

Wanqing Ding¹, Wenbiao Jin¹, Xu Zhou², Shao-Feng Li³, Renjie Tu¹, Song-Fang Han¹, Chuan Chen⁴, Xiaochi Feng¹, Yan Huang¹

Affiliations

¹ Shenzhen Engineering Laboratory of Microalgal Bioenergy, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China.
² Shenzhen Engineering Laboratory of Microalgal Bioenergy, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China. Electronic address: zhouxu@hit.edu.cn.
³ Department of Building and Environmental Engineering, Shenzhen Polytechnic, Shenzhen 518055, China.
⁴ State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.

PMID: 32197194
DOI: 10.1016/j.biortech.2020.123162

Abstract

Herein, a novel innovative lipid co-extraction strategy using the biodiesel-producing microalga Chlorella pyrenoidosa and planktonic cladoceran Daphnia was proposed. Co-extraction occurred as Daphnia ingested and digested microalgal cells in a pre-treatment process; thereafter, lipids from these organisms were extracted. Composition of fatty acids from C. pyrenoidosa and Daphnia were appropriate as potential biodiesel feedstocks. Daphnia had different absorption and conversion capacities of various fatty acids from C. pyrenoidosa, which showed potential for improving biodiesel characteristics. Linoleic acid (LA, C18:2n-6) and alpha-linolenic acid (ALA, C18:3n-3) were absorbed significantly into the body of Daphnia. The optimal lipid extraction and fatty acid methyl esters (FAMEs) recovery rates were up to 41.08% and 12.35%, respectively, which were greater than that of the traditional lipid extraction method due to the rich oil content of Daphnia. Overall, this lipid co-extraction process serves a potential Daphnia utilization as an economical, green, low-energy way for microalgae biodiesel production.

Keywords: Biodiesel production; Chlorella pyrenoidosa; Daphnia; Fatty acid conversion; Lipid extraction.