Developmental exposure to low doses of dichlorodiphenyltrichloroethane impairs proliferative response of thymic lymphocytes to Concanavalin A in rats

Nataliya V Yaglova; Elina S Tsomartova; Sergey S Obernikhin; Marina Y Ivanova; Elizaveta V Chereshneva; Svetlana G Muhamedova; Tatiana A Lomanovskaya; Valentin V Yaglov

doi:10.1016/j.heliyon.2020.e03608

Developmental exposure to low doses of dichlorodiphenyltrichloroethane impairs proliferative response of thymic lymphocytes to Concanavalin A in rats

Heliyon. 2020 Mar 17;6(3):e03608. doi: 10.1016/j.heliyon.2020.e03608. eCollection 2020 Mar.

Authors

Nataliya V Yaglova¹, Elina S Tsomartova², Sergey S Obernikhin¹, Marina Y Ivanova², Elizaveta V Chereshneva², Svetlana G Muhamedova², Tatiana A Lomanovskaya², Valentin V Yaglov¹

Affiliations

¹ Laboratory of Endocrine System Development, Federal State Budgetary Institution Research Institute of Human Morphology, Moscow, Russia.
² Department of Histology, Cytology, and Embryology, Federal State Funded Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University, Moscow, Russia.

Abstract

The aim of the research was to study formation of thymic lymphocytes proliferative response to T cell mitogen Concanavalin A in 7, 42, and 70 days-old male Wistar rats developmentally exposed to low doses of endocrine disruptor dichlorodiphenyltrichloroethane (2.90 ± 0.13 μg/kg body weight). The thymus of the exposed rats did not show morphological abnormalities. Exposure to the endocrine disrupter was found to alter age-dependent changes of thymic lymphocyte proliferative activity and attenuate proliferative response to Concanavalin A in puberty and adulthood. Insufficient response to mitogen was mediated by higher content of actively proliferating Ki-67-positive lymphoblasts compared to the control values. Insufficient proliferative response to mitogen in developmentally exposed to the endocrine disruptor rats may provide higher risk of impaired cellular immune reactions.

Keywords: Cell differentiation; Concanavalin A; Development; Developmental biology; Dichlorodiphenyltrichloroethane; Endocrine disrupter; Immune system; Immunology; Proliferative response; Thymic lymphocytes; Thymus; Toxicology.