Distinct microbial communities and their networks in an anammox coupled with sulfur autotrophic/mixotrophic denitrification system

Shuai Du; Tao Ya; Minglu Zhang; Minghan Zhu; Nankun Li; Shuwei Liu; Xiaohui Wang

doi:10.1016/j.envpol.2020.114190

Distinct microbial communities and their networks in an anammox coupled with sulfur autotrophic/mixotrophic denitrification system

Environ Pollut. 2020 Jul:262:114190. doi: 10.1016/j.envpol.2020.114190. Epub 2020 Mar 2.

Authors

Shuai Du¹, Tao Ya¹, Minglu Zhang², Minghan Zhu¹, Nankun Li³, Shuwei Liu⁴, Xiaohui Wang⁵

Affiliations

¹ Beijing Engineering Research Center of Environmental Material for Water Purification, College of Chemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.
² Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing, 100048, China.
³ Environmental Engineering Evaluation Center, Ministry of Ecology and Environment, Beijing, 100012, China.
⁴ Chongqing Science and Technology Branch, National Power Investment Group Yuanda Environmental Engineering Co., Ltd., Chongqing, 401120, China.
⁵ Beijing Engineering Research Center of Environmental Material for Water Purification, College of Chemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, China. Electronic address: xhwang@mail.buct.edu.cn.

PMID: 32193079
DOI: 10.1016/j.envpol.2020.114190

Abstract

Organ carbon are often used to enhance denitrification in wastewater treatment. However, their possible effects on microbial interactions are very limited. In this work, an anaerobic ammonium oxidation (anammox) coupled with sulfur autotrophic/mixotrophic denitrification (SAD/SMD) system was used to investigate the changes in microbial interactions among the microbial communities under different nutrient condition. The removal efficiency of total nitrogen increased from 70% (SAD) to 97% (SMD). The Illumina sequencing analysis indicated that Planctomycetes was the most dominant bacterial phylum in anammox system. Thiobacillus and Sulfurimonas, two typical autotrophic denitrifiers, decreased significantly from 31.9% to 17.7%-12.2% and 9.3%, when the nutrient condition changed from SAD to SMD (P < 0.05). Meanwhile, some heterotrophic or mixotrophic denitrifying bacteria, including Gemmobacter, Pseudomonas and Thauera increased significantly (P < 0.05). Molecular ecological network (MEN) analysis showed that the addition of organic carbon substantially altered the overall architecture of the network. Compared with SAD, the SMD had shorter path lengths, indicating higher transfer efficiencies of information and materials among different microorganism. The addition of organic carbon increased the microbial interaction complexity of Proteobacteria. The links of Thiobacillus, which was a typical sulfur-oxidizing autotrophic denitrifying bacteria, significantly reduced (P < 0.05) with the addition of organic carbon, while the links of the heterotrophic bacteria Geobacter significantly increased (P < 0.05). This study provided new insights into our understanding of the shifts in the bacteria community and their microbial interactions under different nutrient conditions (SAD and SMD) in sulfur-supported denitrification system.

Keywords: Anammox; MENs analysis; Microbial diversity changes; Nitrogen removal performance; Sulfur-supported denitrification.

MeSH terms

Autotrophic Processes
Bioreactors
Denitrification*
Microbiota*
Nitrates
Nitrogen
Sulfur

Substances

Nitrates
Sulfur
Nitrogen