Four species of the genus Tetrabothrius were reported from three species of Antarctic flying seabirds. There were: Tetrabothrius (Culmenamniculus) gracilis Nybelin, 1916 and Tetrabothrius (Oriana) minutus Szpotańska, 1917 from Macronectes giganteus (Gmelin, 1789); Tetrabothrius (Oriana) filiformis Nybelin, 1916 from Larus dominicanus Lichtenstein, 1823 and Tetrabothrius (Culmenamniculus) cylindraceus Rudolphi, 1849 from Stercorarius antarcticus lonnbergi (Mathews, 1912). M. giganteus is a new host for T. (Culmenamniculus) gracilis and T. (Oriana) minutus, and L. dominicanus is a new host for T. (Oriana) filiformis. The South Shetland Islands are a new locality for T. (Oriana) minutus and T. (Culmenamniculus) gracilis