Objective: HiPS stem cells are commonly used for the study of medical disorders. Thelaboratory in which this study was conducted uses these cells for examining the treatment and cureof neurodegenerative diseases. Bone regeneration poses the greatest challenge for an oral surgeonboth in terms of increased implant osseointegration and reducing bone healing times. The aim ofthis study was to validate the protocol in the literature to produce and then test in vitro osteoblastswith different nanomaterials to simulate bone regeneration.
Method: hiPS clones (#2, #4, and #8)were differentiated into an osteoblast cell culture tested for alizarin red staining and for alkalinephosphatase testing at 14, 21 and 28 days, after the cells were plated.
Results: The cells showeddiffuse positivity under alizarin red staining and the alkaline phosphatase (ALP)-test, showingsmall formations of calcium clusters.
Conclusion: Despite the limitations of our study, it is a startingpoint for further protocols, laying a solid foundation for research in the field of bone regenerationthrough the use of stem cells. .
Keywords: biomaterials; bone regeneration; bone substitutes; growth factor; nanomaterials; stem cells.