Objective: To investigate the inhibitory effect of Jinghua Weikang capsule (JWC) on gastric inflammation induced by Helicobacter pylori (H. pylori) via the nuclear factor-kappa B (NF-¦ÊB) signaling pathway in Kunming mice.
Methods: We investigated the anti-inflammation potential of JWC extract in vivo in a H. pylori-induced gastritis mouse model. The expression of inflammation-related molecules was evaluated by Western blotting, and the concentrations of in vivo inflammatory markers were measured by enzyme-linked immunosorbent assay. Inflammatory cell infiltration was evaluated by histopathological examination, and mRNA levels of related genes were evaluated by quantitative reverse transcription polymerase chain reaction.
Results: JWC had a dose-dependent protective effect against H. pylori-induced gastritis by protecting gastric epithelial cells and inhibiting inflammatory cell infiltration. Mechanistically, JWC decreased the protein levels of phosphorylated I¦ÊB¦Á and NF-¦ÊB p65, mRNA levels of NF-¦ÊB pathway molecules, and plasma levels of tumor necrosis factor-¦Á and interleukin 1 beta.
Conclusion: An important finding of our study is that JWC attenuated gastrointestinal inflammation and ulceration and exerted a protective effect against gastric injury via inhibition of inflammation reactions and regulating the canonical NF-κB signaling pathway in vivo.
Keywords: Helicobacter pylori; Jinghua Weikang capsule; NF-kappa B; Signal transduction.