Objective: To understand the distribution of nerve fibers and the types of neural cells in Aspidogaster conchiola.
Methods: Whole worms were subjected to silver staining, histochemical staining and hematoxylin-eosin (HE) staining, and the nervous systems of the worms were observed.
Results: There were 3 types of neural cells in the worm head near the cerebral ganglion, including unipolar, bipolar and multipolar neurons, which were divided into 7 types according to the morphology. There was a nerve network on the surface of pharynx and intestinal tract, as well as the reproductive organ, including testis, ovary, lower uterus and penis sac. The nerve network was consisted of circular and longitudinal nerve fibers, and the structure of the nerve network around the mouth was similar to central nerve.
Conclusions: The structure of the A. conchiola central nervous system is very complicated, and the neural networks may be associated with the physiologic activity of the worm. Different neural cells may have diverse functions.
［摘要］ 目的 了解贝居腹盾吸虫神经纤维分布及神经细胞类型。方法 采用硝酸银染色法、组织化学技术和HE染色法, 对贝居腹盾吸虫神经系统进行观察。结果 贝居腹盾吸虫前端脑神经节附近存在单极、双极、多极3类神经细胞, 形态上可分为7种不同类型; 该吸虫消化器官咽和肠道以及生殖器官睾丸、卵巢、子宫末段、阴茎囊表面均有神经网分布, 由环形和纵向神经纤维组成; 围绕口腔的神经网与中枢神经结构相似。结论 贝居腹盾吸虫神经系统结构非常复杂, 这些神经可能与吸虫生理活动相关, 不同神经细胞可能承担不同功能。.
Keywords: Aspidogaster conchiola; Central nervous system; Neural cell; Silver staining.