Islet Stellate Cells Regulate Insulin Secretion via Wnt5a in Min6 Cells

Wei Xu; Peter M Jones; Houfa Geng; Rui Li; Xuekui Liu; Yinxia Li; Qian Lv; Ying Liu; Jie Wang; Xiuli Wang; Zilin Sun; Jun Liang

doi:10.1155/2020/4708132

Islet Stellate Cells Regulate Insulin Secretion via Wnt5a in Min6 Cells

Int J Endocrinol. 2020 Feb 24:2020:4708132. doi: 10.1155/2020/4708132. eCollection 2020.

Authors

Wei Xu^{1

2}, Peter M Jones², Houfa Geng¹, Rui Li¹, Xuekui Liu¹, Yinxia Li¹, Qian Lv¹, Ying Liu¹, Jie Wang¹, Xiuli Wang¹, Zilin Sun³, Jun Liang¹

Affiliations

¹ Department of Endocrinology, Xuzhou Central Hospital, Xuzhou Institute of Medical Sciences, Xuzhou Clinical School of Nanjing Medical University, Affiliated Hospital of Medical School of Southeast University, Xuzhou, Jiangsu, China.
² Diabetes Research Group, Division of Diabetes & Nutritional Sciences, School of Medicine, King's College London, London, UK.
³ Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, Medical School, Southeast University, Nanjing, China.

Abstract

Background: Type 2 diabetes mellitus is a serious public health problem worldwide. Accumulating evidence has shown that β-cell dysfunction is an important mechanism underlying diabetes mellitus. The changes in the physiological state of islet stellate cells (ISCs) and the effects of these cells on β-cell dysfunction is an important mechanism underlying diabetes mellitus. The changes in the physiological state of islet stellate cells (ISCs) and the effects of these cells on.

Methods: Glucose-stimulated insulin secretion (GSIS) from Min6 cells was examined by estimating the insulin levels in response to high glucose challenge after culture with ISC supernatant or exogenous Wnt5a. Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) analyses were used to observe changes in the β-cell dysfunction is an important mechanism underlying diabetes mellitus. The changes in the physiological state of islet stellate cells (ISCs) and the effects of these cells on.

Results: We observed a significant increase in insulin secretion from Min6 cells cocultured in vitro with supernatant from db/m mouse ISCs compared to that from Min6 cells cocultured with supernatant from db/db mouse ISCs; The intracellular Ca²⁺ concentration in Min6 cells increased in cultured in vitro with supernatant from db/m mouse ISCs and exogenous Wnt5a compared to that from control Min6 cells. Culture of Min6 cells with exogenous Wnt5a caused a significant increase in pCamKII, pFoxO1, PDX-1, and Glut2 levels compared to those in Min6 cells cultured alone; this treatment further decreased Ror2 and Cask expression but did not affect β-cell dysfunction is an important mechanism underlying diabetes mellitus. The changes in the physiological state of islet stellate cells (ISCs) and the effects of these cells on.

Conclusion: ISCs regulate insulin secretion from Min6 cells through the Wnt5a protein-induced Wnt-calcium and FoxO1-PDX1-GLUT2-insulin signalling cascades.