Detection of mitophagy in mammalian cells, mice, and yeast

Hannah Calvelli; Judith Krigman; Mashun Onishi; Derek P Narendra; Nuo Sun; Koji Okamoto

doi:10.1016/bs.mcb.2019.10.006

Detection of mitophagy in mammalian cells, mice, and yeast

Methods Cell Biol. 2020:155:557-579. doi: 10.1016/bs.mcb.2019.10.006. Epub 2019 Nov 27.

Authors

Hannah Calvelli¹, Judith Krigman², Mashun Onishi³, Derek P Narendra⁴, Nuo Sun⁵, Koji Okamoto⁶

Affiliations

¹ Inherited Movement Disorders Unit, Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, United States.
² Departments of Physiology and Cell Biology, The Ohio State University Wexner Medical Center, Columbus, OH, United States; Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, United States.
³ Laboratory of Mitochondrial Dynamics, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan.
⁴ Inherited Movement Disorders Unit, Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, United States. Electronic address: derek.narendra@nih.gov.
⁵ Departments of Physiology and Cell Biology, The Ohio State University Wexner Medical Center, Columbus, OH, United States; Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, United States. Electronic address: nuo.sun@osumc.edu.
⁶ Laboratory of Mitochondrial Dynamics, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan. Electronic address: kokamoto@fbs.osaka-u.ac.jp.

PMID: 32183977
DOI: 10.1016/bs.mcb.2019.10.006

Abstract

Selective elimination of superfluous or dysfunctional mitochondria is a fundamental process conserved among both uni- and multicellular eukaryotes, contributing to mitochondrial quality and quantity control. This process depends on autophagy, a cellular self-eating membrane trafficking system, and is thus called mitophagy. In this chapter, we describe methods to detect mitophagy in mammalian cells, mice, and yeast.

Keywords: Autophagy; Lysosome; Macroautophagy; Mitochondria; Park2; Park6.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cytological Techniques / methods*
Female
HeLa Cells
Humans
Lysosomes / metabolism
Male
Mice, Transgenic
Mitophagy*
Saccharomyces cerevisiae / metabolism
Ubiquitin / metabolism

Substances

Ubiquitin