Anthocyanin supplementation improves anti-oxidative and anti-inflammatory capacity in a dose-response manner in subjects with dyslipidemia

Hanyue Zhang; Zhongliang Xu; Huiwen Zhao; Xu Wang; Juan Pang; Qing Li; Yan Yang; Wenhua Ling

doi:10.1016/j.redox.2020.101474

Anthocyanin supplementation improves anti-oxidative and anti-inflammatory capacity in a dose-response manner in subjects with dyslipidemia

Redox Biol. 2020 May:32:101474. doi: 10.1016/j.redox.2020.101474. Epub 2020 Feb 26.

Authors

Hanyue Zhang¹, Zhongliang Xu², Huiwen Zhao³, Xu Wang⁴, Juan Pang⁵, Qing Li⁶, Yan Yang⁷, Wenhua Ling⁸

Affiliations

¹ Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong Province, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province, PR China. Electronic address: zhanghy97@mail2.sysu.edu.cn.
² Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong Province, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province, PR China. Electronic address: xuzhliang3@mail2.sysu.edu.cn.
³ Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong Province, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province, PR China. Electronic address: zhaohw5@mail2.sysu.edu.cn.
⁴ Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong Province, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province, PR China. Electronic address: wangxu25@mail2.sysu.edu.cn.
⁵ Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong Province, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province, PR China. Electronic address: pangj7@mail2.sysu.edu.cn.
⁶ Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong Province, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province, PR China. Electronic address: liqing45@mail2.sysu.edu.cn.
⁷ Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province, PR China; Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou, Guangdong Province, PR China; Department of Nutrition, School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong Province, PR China. Electronic address: yangyan3@mail.sysu.edu.cn.
⁸ Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong Province, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province, PR China; Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou, Guangdong Province, PR China. Electronic address: lingwh@mail.sysu.edu.cn.

Abstract

Background: Anthocyanins, one of the major plant bioactive substances, possess anti-oxidative and anti-inflammatory capacity. However, their dose-response relationship has remained unclear. The present study investigated the dose-response relationship of anthocyanins with oxidative stress and inflammation in subjects with dyslipidemia.

Design: and Participants: A total of 169 participants with dyslipidemia were randomly assigned to placebo (n = 43), anthocyanins 40 mg/day (n = 44), 80 mg/day (n = 40), or 320 mg/day (n = 42) groups. Urine 8-iso-prostaglandin F₂_α (8-iso-PGF₂_α), 8-hydroxy-2'-deoxyguanosine (8-OHdG) and serum malonaldehyde (MDA), total superoxide dismutase (T-SOD), UA (uric acid), interleukin (IL)-6, IL-10, tumor necrosis factor-α (TNF-α), and C-reactive protein (CRP) were measured at baseline, at 6 weeks, and at 12 weeks.

Results: Anthocyanin supplementation (320 mg/day) for 6 weeks significantly improved T-SOD versus baseline (P < 0.05). A slight reduction in serum IL-6, TNF-α, and urine 8-iso-PGF₂_α from the baseline was observed at 12 weeks in the group receiving 40 mg/day anthocyanins. Anthocyanins (80 mg/day) significantly reduced serum IL-6 (-20%), TNF-α (-11%) and urine 8-iso-PGF₂_α (-27%) versus baseline (P < 0.05). Moreover, 320 mg/day anthocyanin supplementation reduced serum IL-6 (-40%), TNF-α (-21%), MDA (-20%) and urine 8-iso-PGF₂_α (-37%) and 8-OHdG (-36%) than 80 mg/day and 40 mg/day anthocyanins, P value < 0.05. Anthocyanin supplementation has dose-response relationships with decreased inflammatory cytokines IL-6, TNF-α and oxidative stress biomarkers 8-iso-PGF₂_α, 8-OHdG and MDA (P for trend, <0.05). Furthermore, a strong positive correlation was observed between the changes in the urine 8-iso-PGF₂_α , 8-OHdG levels and serum IL-6 levels in subjects from anthocyanin groups after 12 weeks of treatment.

Conclusions: Supplementation of anthocyanins for 12 weeks positively improved the anti-oxidative and anti-inflammatory capacity in a dose-response manner in individuals with dyslipidemia.

Keywords: 8-Hydroxy-2′-deoxyguanosine; 8-iso-prostaglandinF(2)(α); Anthocyanins; Interleukin-6; Randomized controlled trial.

Publication types

Randomized Controlled Trial
Research Support, Non-U.S. Gov't

MeSH terms

Anthocyanins* / pharmacology
Anti-Inflammatory Agents / pharmacology
Anti-Inflammatory Agents / therapeutic use
Biomarkers
Dietary Supplements
Dyslipidemias* / drug therapy
Humans
Oxidative Stress

Substances

Anthocyanins
Anti-Inflammatory Agents
Biomarkers