Identifying the Cardiac Dyad Proteome In Vivo by a BioID2 Knock-In Strategy

Circulation. 2020 Mar 17;141(11):940-942. doi: 10.1161/CIRCULATIONAHA.119.043434. Epub 2020 Mar 16.

Authors

Wei Feng¹, Canzhao Liu¹, Simone Spinozzi¹, Li Wang², Sylvia M Evans^{1

3}, Ju Chen

Affiliations

¹ Department of Medicine (W.F., C.L., S.S., S.M.E.), University of California, San Diego, La Jolla.
² Division of Biological Sciences (L.W.), University of California, San Diego, La Jolla.
³ Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences (S.M.E.), University of California, San Diego, La Jolla.

No abstract available

Keywords: cardiac dyad; proximity labeling; transverse-tubule.

Publication types

Letter
Research Support, N.I.H., Extramural

MeSH terms

Animals
Biotinylation
CRISPR-Cas Systems
Excitation Contraction Coupling / genetics*
Gene Editing
Gene Knock-In Techniques / methods*
Gene Ontology
Membrane Proteins / genetics
Membrane Proteins / metabolism
Membrane Proteins / physiology*
Mice
Muscle Proteins / genetics
Muscle Proteins / metabolism
Muscle Proteins / physiology*
Myocytes, Cardiac / physiology*
Oligopeptides
Protein Interaction Mapping
Proteome*
Recombinant Fusion Proteins / metabolism
Sarcoplasmic Reticulum / metabolism
Tandem Mass Spectrometry

Substances

Membrane Proteins
Muscle Proteins
Oligopeptides
Proteome
Recombinant Fusion Proteins
junctophilin-2 protein, mouse
FLAG peptide

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