Dynamics and response of microbial diversity to nutritional conditions in denitrifying bioreactor for linear alkylbenzene sulfonate removal

Marcus Vinicius Freire Andrade; Tiago Palladino Delforno; Isabel Kimiko Sakamoto; Edson Luiz Silva; Maria Bernadete Amâncio Varesche

doi:10.1016/j.jenvman.2020.110387

Dynamics and response of microbial diversity to nutritional conditions in denitrifying bioreactor for linear alkylbenzene sulfonate removal

J Environ Manage. 2020 Jun 1:263:110387. doi: 10.1016/j.jenvman.2020.110387. Epub 2020 Mar 9.

Authors

Marcus Vinicius Freire Andrade¹, Tiago Palladino Delforno², Isabel Kimiko Sakamoto³, Edson Luiz Silva⁴, Maria Bernadete Amâncio Varesche³

Affiliations

¹ Federal Institute of Education, Science and Technology of Ceará, IFCE, Campus Sobral, 62042-030, Sobral, CE, Brazil. Electronic address: marcus.andrade@ifce.edu.br.
² Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, Campinas, 13081-970, SP, Brazil.
³ Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Trabalhador São-carlense, 13566-590, São Carlos, SP, Brazil.
⁴ Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luiz, Km 235, SP 310, 13565-905, São Carlos, SP, Brazil.

PMID: 32174528
DOI: 10.1016/j.jenvman.2020.110387

Abstract

The aim of this study was to evaluate the microbial structure and phylogenetic diversity under the influence of nutritional conditions and hydraulic retention time (HRT) in fluidized bed reactors (FBR), operated in short HRT (8 h - FBR8; 12 h - FBR12) for linear alkylbenzene sulfonate (LAS) removal from laundry wastewater. After each phase, biofilm samples from FBR8 and FBR12 were submitted to microbial sequencing by Mi-Seq Illumina®. Higher LAS removal rates were observed after 313 days, achieving 99 ± 3% in FBR12 (22.5 ± 5.9 mg LAS/L affluent) and 93 ± 12% in FBR8 (20.6 ± 4.4 mg LAS/L affluent). Different modifications involving genera of bacteria were observed throughout the reactors operation. The identified microorganisms were, mostly, related to LAS degradation and nitrogen conversion such as Dechloromonas, Flavobacterium, Pseudomonas, and Zoogloea.

Keywords: Denitrification; Ethanol; Hydraulic retention time; Nitrate; Stratification.

MeSH terms

Alkanesulfonic Acids*
Biodegradation, Environmental
Bioreactors*
Denitrification
Phylogeny
Wastewater

Substances

Alkanesulfonic Acids
Waste Water
alkylbenzyl sulfonic acid