Construction and structure-activity mechanism of polysaccharide nano-selenium carrier

Shuqian Hu; Weicheng Hu; Yanru Li; Shujing Li; Huafeng Tian; Ang Lu; Jianguo Wang

doi:10.1016/j.carbpol.2020.116052

Construction and structure-activity mechanism of polysaccharide nano-selenium carrier

Carbohydr Polym. 2020 May 15:236:116052. doi: 10.1016/j.carbpol.2020.116052. Epub 2020 Feb 21.

Authors

Shuqian Hu¹, Weicheng Hu², Yanru Li¹, Shujing Li³, Huafeng Tian⁴, Ang Lu⁵, Jianguo Wang⁶

Affiliations

¹ College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
² Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China.
³ Beijing Key Lab of Plant Resource Research and Development, Beijing Technology and Business University, Beijing 100048, China.
⁴ Beijing Key Laboratory of Quality Evaluation Technology for Hygiene and Safety of Plastics, Beijing Technology and Business University (BTBU), Beijing 100048, China.
⁵ College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
⁶ College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: wjg5118@nwafu.edu.cn.

PMID: 32172867
DOI: 10.1016/j.carbpol.2020.116052

Abstract

In this study, a series water soluble sulfate polysaccharides (SPS) with different degrees of substitutions (DS = 0.02∼0.28) were prepared using a linear water-insoluble β-d-(1→3)-glucan. SPS-1, SPS-3 and SPS-7 with substitution degrees of 0.02, 0.06 and 0.25 were used as templates to prepare stable and size controlled selenium nanoparticles (SeNPs) with diameter from 54.35 to 123.04 nm using one-step method. The selenium contents of SPS-SeNPs with large, medium and small sizes were 0.172 %, 0.274 % and 0.305 %, respectively. SPS-SeNPs was confirmed to inhibit the production of nitric oxide (NO) in RAW 264.7 inflammatory macrophages induced by lipopolysaccharide (LPS), and downregulated the mRNA expression of TNF-α, IL-1β and iNOS. The results indicated that SPS-SeNPs had significant anti-inflammatory activity. Moreover, SPS-SeNPs with smaller size showed higher anti-inflammatory effects. All of these results suggest that SeNPs-SPS carriers have anti-inflammatory in concentration-dependent and size-dependent model.

Keywords: Anti-inflammatory activity; Polysaccharides; Selenium nanoparticles; Structure-activity relationship.