Trace elements play an indispensable role in stabilizing the performance of anaerobic co-digestion (Co-AD) of food waste (FW) and sewage sludge (SS) at greater organic load (OL). The results of high organic-loaded reactors showed that the stability of the system failed due to the buildup of volatile fatty acid (VFA) and ammonia. At the OL of 6.5 g/L, the stability of the system failed due to the buildup of propionic acid. The optimum dosage of Fe (5000 mg/L), Ni (200 mg/L), Zn (320 mg/L), and Mo (2.2 mg/L) was experimentally determined and added to reduce the inhibition condition. Consequently, the propionic acid concentration, which was above 1500 mg/L reduced to under 500 mg/L during Co-AD. Hence, higher biogas production, and biodegradability of 236 ± 23 mL/g VS, and 41.75%, respectively, were obtained. Increasing OL (9.5 g/L), the stability of the system was hindered due to only the buildup of ammonia (up to 188 ± 6 NH3-N mg/L). Therefore, the trace elements of Cu (250 mg/L) and Co (3 mg/L) were experimentally determined and added into the Co-AD to diminish ammonia accumulation and process instability. The experimental results showed that at OL of 14 g/L, biogas production, low ammonia concentration and biodegradability of 332 ± 21 mL/g VS, and 70 NH3-N mg/L, and 57.89%, respectively, were achieved. However, the performance and stability of the system failed at the higher OL due to the more increased ammonia and VFA concentration, and the greater dosages of trace elements did not enhance the process stability.
Keywords: Biogas production; Co-substrate; Co-trace elements; Inhibitory agents; VFA and ammonia inhibition.