Investigation of heterogeneous sulfonated graphene oxide to hydrolyze cellulose and produce dark fermentative biohydrogen using Enterobacter aerogenes

Hadiseh Tondro; Hamid Zilouei; Kiomars Zargoosh; Mehdi Bazarganipour

doi:10.1016/j.biortech.2020.123124

Investigation of heterogeneous sulfonated graphene oxide to hydrolyze cellulose and produce dark fermentative biohydrogen using Enterobacter aerogenes

Bioresour Technol. 2020 Jun:306:123124. doi: 10.1016/j.biortech.2020.123124. Epub 2020 Mar 4.

Authors

Hadiseh Tondro¹, Hamid Zilouei², Kiomars Zargoosh³, Mehdi Bazarganipour⁴

Affiliations

¹ Department of Chemical Engineering, Isfahan University of Technology, Isfahan 8415683111, Iran.
² Department of Chemical Engineering, Isfahan University of Technology, Isfahan 8415683111, Iran. Electronic address: hzilouei@cc.iut.ac.ir.
³ Department of Chemistry, Isfahan University of Technology, Isfahan 8415683111, Iran.
⁴ Research Institute for Nanotechnology and Advanced Materials, Isfahan University of Technology, Isfahan 8415683111, Iran.

PMID: 32172093
DOI: 10.1016/j.biortech.2020.123124

Abstract

The main aim of this work was investigating the potential of sulfonated graphene oxide (sGO) for hydrolysis of cellulosic substrates and dark fermentative hydrogen production from obtained hydrolysates using E. aerogenes. Sulfonation of graphene oxide was performed using chlorosulfonic acid which showed a high acid density of 4.63 mmol/g. Influence of the reaction time (1-5 h), temperature (90-180 °C) and sGO dosage (62.5-500 mg in 25 mL reaction volume) on the hydrolysis of pretreated microcrystalline cellulose was experimented. It revealed that the yield of glucose and total reducing sugars and selectivity can reach 454.4 ± 22.20 mg/g, 682.6 ± 30.67 mg/g and 95.5%, respectively, at 150 °C for 3 h using 250 mg sGO. The maximum hydrogen efficiency of 150.0 ± 5.65 mL/g was achieved under optimized conditions, which was 2.2-fold higher than that from the pretreated MCC substrate as control in the absence of sGO (67.3 ± 8.84 mL/g).

Keywords: Biohydrogen; Dark fermentation; Enterobacter aerogenes; Response surface methodology; Solid acid catalytic hydrolysis; Sulfonated graphene oxide.