Strigolactone mediates jasmonic acid-induced lipid production in microalga Monoraphidium sp. QLY-1 under nitrogen deficiency conditions

Xueting Song; Yongteng Zhao; Benyong Han; Tao Li; Peng Zhao; Jun-Wei Xu; Xuya Yu

doi:10.1016/j.biortech.2020.123107

Strigolactone mediates jasmonic acid-induced lipid production in microalga Monoraphidium sp. QLY-1 under nitrogen deficiency conditions

Bioresour Technol. 2020 Jun:306:123107. doi: 10.1016/j.biortech.2020.123107. Epub 2020 Mar 3.

Authors

Xueting Song¹, Yongteng Zhao¹, Benyong Han¹, Tao Li¹, Peng Zhao¹, Jun-Wei Xu¹, Xuya Yu²

Affiliations

¹ Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
² Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. Electronic address: xuya_yu@163.com.

PMID: 32172089
DOI: 10.1016/j.biortech.2020.123107

Abstract

The roles of jasmonic acid (JA) in the regulation of cell growth and lipid biosynthesis under the combination of strigolactone (SL) treatment and nitrogen deficiency (ND) were investigated. In this work, the optimised ND condition (46.18%) and ND combined with SL treatment (53.71%) showed 1.11- and 1.29-fold increases in lipid content in Monoraphidium sp. QLY-1 compared with the control condition (41.57%). The levels of JA, glutathione (GSH), and γ-aminobutyric acid (GABA) and lipogenic genes expression were upregulated by the combination of SL and ND, but the ROS level was decreased. Furthermore, exogenous JA supplementation induced the highest lipid content (57.12%) and productivity (312.35 mg L^-1 d^-1) under ND combined with SL treatment. This study provided a combined strategy for enhancing lipid production and supplied novel insights into the role of JA signalling in regulating lipid synthesis and oxidative stress in microalgae by combining SL treatment with ND.

Keywords: Jasmonic acid; Lipid; Monoraphidium sp. QLY-1; Nitrogen deficiency; Strigolactone.