Conducting Influenza Virus Pathogenesis Studies in Avian Species

Carol J Cardona; David A Halvorson; Jeffrey Hall; Mary J Pantin-Jackwood; Justin D Brown

doi:10.1007/978-1-0716-0346-8_15

Conducting Influenza Virus Pathogenesis Studies in Avian Species

Methods Mol Biol. 2020:2123:195-209. doi: 10.1007/978-1-0716-0346-8_15.

Authors

Carol J Cardona¹, David A Halvorson², Jeffrey Hall³, Mary J Pantin-Jackwood⁴, Justin D Brown⁵

Affiliations

¹ Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA. ccardona@umn.edu.
² Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
³ National Wildlife Health Center, Madison, WI, USA.
⁴ Exotic and Emerging Avian Viral Diseases Unit, US National Poultry Research Center, US Department of Agriculture, Agricultural Research Service, Athens, GA, USA.
⁵ Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, State College, PA, USA.

PMID: 32170690
DOI: 10.1007/978-1-0716-0346-8_15

Abstract

Avian infection studies with influenza A are an important means of assessing host susceptibility, viral pathogenesis, host responses to infection, mechanisms of transmission, viral pathotype, and viral evolution. Complex systems and natural settings may also be explored with carefully designed infection studies. In this chapter, we explore the elements of infection studies, general guidelines for choosing a virus to use, host selection, and many aspects of study design.

Keywords: Avian; Challenge; Host; Infection; Influenza; Inoculation; Pathogenesis; Sample; Shedding; Transmission.

Publication types

Review

MeSH terms

Animals
Birds / virology*
Housing, Animal
Influenza A virus / pathogenicity*
Influenza in Birds / virology*
Specimen Handling
Virology / methods*
Virus Shedding