"French fries"-like luminescent metal organic frameworks for the fluorescence determination of cytochrome c released by apoptotic cells and screening of anticancer drug activity

Ruirui Xie; Yanyan Liu; Peipei Yang; Lan Huang; Xun Zou; Jiamin Liu; Qingfan Ren; Jia Tao; Peng Zhao

doi:10.1007/s00604-020-4207-x

"French fries"-like luminescent metal organic frameworks for the fluorescence determination of cytochrome c released by apoptotic cells and screening of anticancer drug activity

Mikrochim Acta. 2020 Mar 12;187(4):221. doi: 10.1007/s00604-020-4207-x.

Authors

Ruirui Xie¹, Yanyan Liu¹, Peipei Yang², Lan Huang¹, Xun Zou¹, Jiamin Liu¹, Qingfan Ren², Jia Tao³, Peng Zhao⁴

Affiliations

¹ Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, Guangdong, China.
² School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, 510641, Guangdong, China.
³ School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, 510641, Guangdong, China. cejtao@scut.edu.cn.
⁴ Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, Guangdong, China. smuzp@smu.edu.cn.

PMID: 32166378
DOI: 10.1007/s00604-020-4207-x

Abstract

A luminescent metal organic framework was prepared by encapsulating Zn-Ag-In-S quantum dots into "French fries"-like MIL-68(In) metal organic frameworks (ZAISQDs@MIL-68(In)). The ZAISQDs@MIL-68(In) had a maximum excitation wavelength at 370 nm and maximum emission wavelength at 620 nm. It was found that the ZAISQDs@MIL-68(In) was efficiently quenched by cytochrome c (Cyt c), which is an important biomarker of early cell apoptosis. The quenching mechanism was ascribed to be an inner filter effect and dynamic quenching of Cyt c towards the ZAISQDs@MIL-68(In), and the enrichment effect of MIL-68(In). Benefiting from the multiple advantages, ZAISQDs@MIL-68(In) was developed as an assay strategy of Cyt c with logarithmic relation between signal quenching and concentration in the range 0.02 to 3.5 μM. The linear equation was (F₀-F)/F₀ = 0.5043 + 0.2678 × logc_{Cyt c} with a detection limit of 8 nM. Cyt c released by drug induced apoptotic cells was determined by ZAISQDs@MIL-68(In), and this strategy has been utilized for the screening of anticancer drug activity. Graphical abstract Schematic representation of the synthesis of ZAISQDs@MIL-68(In) and its application for Cyt c and screening anticancer drug activity.

Keywords: Anticancer drug; Apoptosis; Cytochrome c; Fluorescence; Metal organic framework; Quantum dots.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents / chemistry*
Antineoplastic Agents / pharmacology
Apoptosis* / drug effects
Cytochromes c / analysis*
Cytochromes c / metabolism
Drug Evaluation, Preclinical
Fluorescence*
HeLa Cells
Humans
Luminescent Agents / chemistry*
Metal-Organic Frameworks / chemistry*
Molecular Structure
Tumor Cells, Cultured

Substances

Antineoplastic Agents
Luminescent Agents
Metal-Organic Frameworks
Cytochromes c

Grants and funding

21705050/Natural Science Foundation of China/International