Beauvericin and ochratoxin A mycotoxins individually and combined in HepG2 cells alter lipid peroxidation, levels of reactive oxygen species and glutathione

Abstract

The co-presence of more than one mycotoxin in food is being evidenced in last food surveys as reported in the literature. Beauvericin (BEA) is a non-legislated emergent mycotoxin while Ochratoxin A (OTA) has been widely studied and legislated. Concentration range individually studied was from 2.5 to 0.3 μM for BEA and from 25 to 3.1 μM for OTA; binary mixture [BEA + OTA] comprised concentrations of 1:10 ratio from [2.5 + 25] to [3.1 + 0.3] μM. Potential of toxicity of BEA in HepG2 cells was the highest at all times assayed (24, 48 and 72h). LPO was performed through malondyaldehyde (MDA) detection denoting in the binary mixture for [1.25 + 12.5] μM and at 24 and 72h the highest disturbance values. ROS denoted differences respect to the control at different times specially for OTA, while in binary combination only for few point times was denoted. Effects detected for ROS and LPO were connected with alterations detected for glutathione levels of oxidized and reduced form. A real scenario of consumers chronically exposed to different mycotoxins and their mixtures is here presented highlighting the good methodology to assess the risk from exposure to combinations of chemicals in food.

Keywords: Glutathione; HepG2 cells; LPO; ROS; mycotoxins’ mixture.