Surface properties and bacterial adhesion of bulk-fill composite resins

Dilber Bilgili; Ayşe Dündar; Çağatay Barutçugil; Deniz Tayfun; Özlem Koyuncu Özyurt

doi:10.1016/j.jdent.2020.103317

Surface properties and bacterial adhesion of bulk-fill composite resins

J Dent. 2020 Apr:95:103317. doi: 10.1016/j.jdent.2020.103317. Epub 2020 Mar 9.

Authors

Dilber Bilgili¹, Ayşe Dündar², Çağatay Barutçugil³, Deniz Tayfun¹, Özlem Koyuncu Özyurt⁴

Affiliations

¹ DDS, Private Office, Antalya, Turkey.
² Department of Restorative Dentistry, Faculty of Dentistry, Akdeniz University, Antalya, Turkey.
³ Department of Restorative Dentistry, Faculty of Dentistry, Akdeniz University, Antalya, Turkey. Electronic address: cagatay@akdeniz.edu.tr.
⁴ Department of Medical Microbiology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.

PMID: 32165185
DOI: 10.1016/j.jdent.2020.103317

Abstract

Objectives: The aim of the present study was to evaluate the Streptococcus mutans and Streptococcus mitis adhesion and related surface properties of bulk-fill resin composite.

Methods: Four novel bulk-fill composite with different composition were used; Sonic Fill-2 (KSF), Filtek BulkFill (FBF), Admira Fusion X-tra (AFX), Beautifil Bulk Restorative (SBB) and a control group (glass) were included in the study. After standardized surface polishing procedure, surface properties of composite specimens were evaluated using surface roughness (SR) measurements by a profilometer, hydrophobicity and surface free energy (SFE) analyses, elemental and topographic analyses by SEM-EDS. To evaluate the bacterial adhesion, composite specimens were immersed in artificial saliva and mucin for pellicle development. After 1-h immersion, bacterial suspension was added to the pellicle-coated specimens, which were incubated at 37 °C in 5% CO₂ atmosphere for 24 h. Adhered bacteria counts were determined as x10⁸ Cfu/ml. Bacterial adhesion was also investigated using confocal laser scanning microscopy.

Results: No statistically significant differences were found among bulk fill composites in terms of surface roughness while glass showed the lowest Ra values. The lowest contact angle values were found in the control group and Sonic Fill-2 while the highest SFE values were observed in these materials. No statistically significant differences were found between the S. mutans counts. For S. Mitis adhesion, the highest value was found in Sonic Fill-2 and no significant differences were observed between the other groups.

Conclusions: SR of bulk-fill composite resins had no effect on bacterial adhesion. However, bacterial adhesion increased with higher SFE values.

Clinical significance: Although the surface roughness of composites used in the study is similar, in clinically, S. mitis adhesion may be more in the KSF group because of high surface free energy.

Keywords: Bacterial adhesion; Bulk-fill; CLSM; Hydrophobicity; Surface free energy; Surface roughness.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Adhesion*
Composite Resins*
Materials Testing
Streptococcus mutans
Surface Properties

Substances

Composite Resins