Optimizing real swine wastewater treatment efficiency and carbohydrate productivity of newly microalga Chlamydomonas sp. QWY37 used for cell-displayed bioethanol production

Wenying Qu; Pau Loke Show; Tomohisa Hasunuma; Shih-Hsin Ho

doi:10.1016/j.biortech.2020.123072

Optimizing real swine wastewater treatment efficiency and carbohydrate productivity of newly microalga Chlamydomonas sp. QWY37 used for cell-displayed bioethanol production

Bioresour Technol. 2020 Jun:305:123072. doi: 10.1016/j.biortech.2020.123072. Epub 2020 Feb 22.

Authors

Wenying Qu¹, Pau Loke Show², Tomohisa Hasunuma³, Shih-Hsin Ho⁴

Affiliations

¹ State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, PR China.
² Department of Chemical Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, 43500 Selangor Darul Ehsan, Malaysia.
³ Graduate School of Science, Technology, and Innovation, Kobe University, Kobe 657-8501, Japan.
⁴ State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, PR China. Electronic address: stephen6949@hit.edu.cnstep.

PMID: 32163881
DOI: 10.1016/j.biortech.2020.123072

Abstract

This work aimed to study an newly isolated microalgal strain, Chlamydomonas sp. QWY37, that can achieve a maximum carbohydrate production of 944 mg/L·d, along with high pollutant removal efficiencies (chemical oxygen demand: 81%, total nitrogen: 96%, total phosphate: nearly 100%) by optimizing culture conditions and using an appropriate operation strategy. Through a cell-displayed technology that utilizes combined engineered system, a maximum microalgal bioethanol yield of 61 g/L was achieved. This is the first report demonstrating the highest microalgal carbohydrate productivity using swine wastewater without any pretreatments associated with direct high-density bioethanol production from the subsequent microalgal biomass. This work may represent a breakthrough in achieving feasible microalgal bioethanol conversion from real swine wastewater.

Keywords: Bioethanol; Carbohydrate; Cell-displayed technology; Microalgae; Real swine wastewater.