High sPLA2-IIA level is associated with eicosanoid metabolism in patients with bacterial sepsis syndrome

PLoS One. 2020 Mar 11;15(3):e0230285. doi: 10.1371/journal.pone.0230285. eCollection 2020.

Abstract

The aim of this study was to determine the association between secretory phospholipase A2 group IIA (sPLA2-IIA) and eicosanoid pathway metabolites in patients with bacterial sepsis syndrome (BSS). Levels of sPLA2-IIA, eicosanoids prostaglandin (PG)E2, PGD synthase were quantified in the sera from patients confirmed to have bacterial sepsis (BS; N = 45), bacterial severe sepsis/septic shock (BSS/SS; N = 35) and healthy subjects (N = 45). Cyclooxygenase (COX)-1 and COX-2 activities were analyzed from cell lysate. Serum levels of sPLA2-IIA, PGE2, and PGDS increased significantly in patients with BS and BSS/SS compared to healthy subjects (p<0.05). COX-2 activity was significantly increased in patients with BS compared to healthy subjects (p<0.05), but not COX-1 activity. Binary logistic regression analysis showed that sPLA2-IIA and PGE2 were independent factors predicting BSS severity. In conclusion, high level of sPLA2-IIA is associated with eicosanoid metabolism in patients with BSS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Bacteremia / blood*
  • Bacteremia / pathology
  • Biomarkers / blood
  • Cyclooxygenase 1 / blood
  • Cyclooxygenase 2 / blood
  • Dinoprostone / blood*
  • Female
  • Group II Phospholipases A2 / blood*
  • Humans
  • Intramolecular Oxidoreductases / blood
  • Lipocalins / blood
  • Male
  • Middle Aged

Substances

  • Biomarkers
  • Lipocalins
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • Group II Phospholipases A2
  • Intramolecular Oxidoreductases
  • prostaglandin R2 D-isomerase
  • Dinoprostone

Grants and funding

This study was supported by the Fundamental Research Grant Scheme FRGS/1/2014/SKK01/UKM/03/3 from the Ministry of Education, Malaysia. The corresponding author (TLT) was the grant holder. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.